The Functions of Klarsicht and Nuclear Lamin in Developmentally Regulated Nuclear Migrations of Photoreceptor Cells in theDrosophilaEye
| Autor: | Janice A. Fischer, Ari B. Molofsky, Christina Robinson, Kristin D. Patterson, Courtney Cater, Shelley Acosta |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
animal structures
Active Transport Cell Nucleus Biology Eye Microtubule medicine Animals Drosophila Proteins Nuclear Matrix Molecular Biology Cell Nucleus Genetics Nesprin Gene Expression Regulation Developmental Membrane Transport Proteins Microtubule organizing center Articles Cell Biology Lamins Cell biology Cell nucleus medicine.anatomical_structure Nuclear lamina Drosophila Photoreceptor Cells Invertebrate SUN domain Nucleus Microtubule-Organizing Center Lamin |
| Zdroj: | Molecular Biology of the Cell. 15:600-610 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e03-06-0374 |
| Popis: | Photoreceptor nuclei in the Drosophila eye undergo developmentally regulated migrations. Nuclear migration is known to require the perinuclear protein Klarsicht, but the function of Klarsicht has been obscure. Here, we show that Klarsicht is required for connecting the microtubule organizing center (MTOC) to the nucleus. In addition, in a genetic screen for klarsicht-interacting genes, we identified Lam Dm0, which encodes nuclear lamin. We find that, like Klarsicht, lamin is required for photoreceptor nuclear migration and for nuclear attachment to the MTOC. Moreover, perinuclear localization of Klarsicht requires lamin. We propose that nuclear migration requires linkage of the MTOC to the nucleus through an interaction between microtubules, Klarsicht, and lamin. The Klarsicht/lamin interaction provides a framework for understanding the mechanistic basis of human laminopathies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|