[Evaluation of the assay technique for detection of anti-chlamydial IgA and IgG antibodies in PID patients]
| Autor: | Y. Tada, Tomohiro Kashiwagi, Yayoi Higashi, Yoichiro Fujiwara, Hideo Honjo, Nobuhito Kashiwagi, Takao A. Yamamoto, Jinsuke Yasuda |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Immunoglobulin A
medicine.medical_specialty Chlamydia trachomatis Enzyme-Linked Immunosorbent Assay medicine.disease_cause Immunoglobulin D Gastroenterology Pregnancy Internal medicine parasitic diseases Pelvic inflammatory disease Medicine Humans Chlamydia biology business.industry General Medicine medicine.disease Assay technique Antibodies Bacterial Evaluation Studies as Topic biology.protein Female Reagent Kits Diagnostic Antibody business Pelvic Inflammatory Disease |
| Zdroj: | Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases. 71(3) |
| ISSN: | 0387-5911 |
| Popis: | The purpose of this study is to evaluate the usefulness and limitation of Rapizyme CHLAMYDIA, enzyme-linked immunosorbent assay (ELISA) for qualitative detection of anti-chlamydial IgG and IgA antibodies, in the serum of 92 PID patients and 73 pregnant women, compared with those of Sero IPALISA CHLAMYDIA. The result of Rapizyme analysis was obtained within 10 minutes with no special devices. Overall agreements of Rapizyme and Sero IPALISA were 90.9% (IgG) and 90.3% (IgA) in the total patients, 88.0% (IgG) and 85.9% (IgA) in PID patients, and 94.5% (IgG) and 95.9% (IgA) in pregnant women. The positive rate of Chlamydia in PID was 17.4% (16/92). Positive agreement of Rapizyme in Chlamydia positive PID and pregnant women was 100% in both IgG and IgA, and negative agreement was also 100%. Positive agreement in Chlamydia negative PID was 100% in both IgG and IgA, and negative agreement was 90.0% (IgG) and 83.3% (IgA). The results of Rapizyme were in close agreement with those of Sero IPALISA. COI (cut off index) of Sero IPALISA clearly decreased in 3 of 6 PID patients during a 3 to 6 months period after chemotherapy, but those changes were not observed in Rapizyme. These results suggest that Rapizyme CHLAMYDIA is a useful diagnostic kit for Chlamydial PID of outpatients. |
| Databáze: | OpenAIRE |
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