A combination of NMR and liquid chromatography to characterize the protective effects of Rhus tripartita extracts on ethanol-induced toxicity and inflammation on intestinal cells

Autor: Hanène Ben Miled, Konstantinos Grintzalis, Sakly Mohsen, Zaineb Ben Barka, Madeleine Polet, Khémais Ben Rhouma, Ulf Sommer, Clement Heude, Yves-Jacques Schneider, Olfa Tebourbi
Přispěvatelé: UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Rok vydání: 2016
Předmět:
0301 basic medicine
Antioxidant
Magnetic Resonance Spectroscopy
medicine.medical_treatment
Clinical Biochemistry
Anti-Inflammatory Agents
Pharmaceutical Science
Plant Roots
Antioxidants
Analytical Chemistry
0302 clinical medicine
Intestinal mucosa
Liquid chromatography–mass spectrometry
Drug Discovery
Intestinal Mucosa
Cytotoxicity
Spectroscopy
Glutathione Transferase
Plant Stems
Chemistry
Enteritis
Intestines
Biochemistry
030220 oncology & carcinogenesis
Toxicity
medicine.symptom
medicine.drug_class
Rhus
Inflammation
LC–MS
Anti-inflammatory
03 medical and health sciences
medicine
Humans
Sulfhydryl Compounds
Plants
Medicinal
Dose-Response Relationship
Drug
Ethanol
Plant Extracts
Interleukin-8
NMR
Ethanol toxicity
Plant Leaves
Oxidative Stress
030104 developmental biology
Cell culture
Cytoprotection
Rhus tripartita extracts
Caco-2 Cells
Chromatography
Liquid
Phytotherapy
Zdroj: Journal of pharmaceutical and biomedical analysis, Vol. 150, p. 347-354 (2018)
ISSN: 1873-264X
Popis: Consumption of ethanol may have severe effects on human organs and tissues and lead to acute and chronic inflammation of internal organs. The present study aims at investigating the potential protective effects of three different extracts prepared from the leaves, root, and stem of the sumac, Rhus tripartita, against ethanol-induced toxicity and inflammation using intestinal cells as a cell culture system, in vitro model of the intestinal mucosa. The results showed an induction of cytotoxicity by ethanol, which was partially reversed by co-administration of the plant extracts. As part of investigating the cellular response and the mechanism of toxicity, the role of reduced thiols and glutathione-S-transferases were assessed. In addition, intestinal cells were artificially imposed to an inflammation state and the anti-inflammatory effect of the extracts was estimated by determination of interleukin-8. Finally, a detailed characterization of the contents of the three plant extracts by high resolution Nuclear Magnetic Resonance (NMR) spectroscopy and mass spectrometry revealed significant differences in their chemical compositions.
Databáze: OpenAIRE
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