Effects of extender, cryoprotectants and thawing protocol on motility of frozen-thawed stallion sperm that were refrozen for intracytoplasmic sperm injection doses
| Autor: | Elaine M. Carnevale, James K. Graham, J. M. Trentin, Raul A. Gonzalez-Castro |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Glycerol
Male food.ingredient Cryoprotectant medicine.medical_treatment Motility Intracytoplasmic sperm injection Cryopreservation law.invention Andrology 03 medical and health sciences 0302 clinical medicine food Cryoprotective Agents Food Animals law Yolk Freezing medicine Animals Horses Sperm Injections Intracytoplasmic Small Animals reproductive and urinary physiology Sperm motility 030219 obstetrics & reproductive medicine urogenital system Equine Chemistry Extender 0402 animal and dairy science 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Spermatozoa Milk embryonic structures Sperm Motility Animal Science and Zoology Semen Preservation |
| Zdroj: | Theriogenology. 136 |
| ISSN: | 1879-3231 |
| Popis: | We examined the effects of different freezing extenders, cryoprotectant agents (CPA) and initial thawing temperatures for preparing doses of refrozen stallion sperm for intracytoplasmic sperm injection (ICSI). Single ejaculates, from twelve stallions, were frozen in lactose-EDTA-egg yolk extender (LE) with 5% glycerol. In experiment 1, sperm were initially thawed to 5 °C or 37 °C, before being diluted in LE or skim milk-egg yolk extender (SMEY) containing either 5% glycerol (GLY), 5% methylformamide (MF) or 5% of a combination of both (GMF). In experiment 2, frozen sperm were initially thawed to 5 °C, diluted and refrozen in SMEY containing 2, 4, 6 or 8% GLY or GMF. In Experiment 1, sperm motility was reduced after each cryopreservation cycle (P 0.05). Extender type did not affect motility after refreezing (P 0.05), but sperm initially thawed to 5 °C exhibited higher motility than sperm thawed to 37 °C (P 0.05). In addition, sperm refrozen in SMEY containing MF or GMF exhibited higher motility than sperm refrozen in GLY alone (P 0.05). In experiment 2, there was an interaction between CPA and CPA concentration (P 0.05). Sperm refrozen with GMF had higher motility than refrozen sperm with GLY (P 0.05), and while GLY concentration did not affect post-thaw motility (P 0.05). Sperm refrozen with 6 or 8% GMF exhibited the highest motility (P 0.05). In conclusion, sperm motility is best maintained when thawing and refreezing stallion sperm in low sperm concentration ICSI doses by initially thawing the sperm to 5 °C and diluting the sperm in a freezing extender with 8% GMF. |
| Databáze: | OpenAIRE |
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