PCR-diagnosis of Anaplasma marginale in cattle populations of Ecuador and its molecular identification through sequencing of ribosomal 16S fragments
| Autor: | Armando Reyna-Bello, Leandro Tana-Hernández, Jorge Ron-Román, Katherine Navarrete-Arroyo, María Augusta Chávez-Larrea |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Veterinary medicine Anaplasmosis Anaplasma 040301 veterinary sciences Population Cattle Diseases Polymerase Chain Reaction 0403 veterinary science 03 medical and health sciences RNA Ribosomal 16S Animals 16S rRNA education Gene Dairy cattle Cloning education.field_of_study lcsh:Veterinary medicine General Veterinary biology 04 agricultural and veterinary sciences General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Bovine disease Anaplasma marginale Bovine Anaplasmosis msp5 030104 developmental biology Rickettsia GenBank lcsh:SF600-1100 Cattle Female Ecuador Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 13, Iss 1, Pp 1-7 (2017) |
| ISSN: | 1746-6148 |
| Popis: | Background Bovine anaplasmosis is an endemic disease in tropical and subtropical areas. It is caused by a bacterium named Anaplasma marginale, and represents an economic problem for cattle farmers due to the losses it generates, such as: mortalities, reduced production, quarantine measures, treatments and control of vectors. The method most often used to diagnose this haemotrophic bacterium is direct examination on blood smear, which sensitivity and specificity are limited compared to other methods such as PCR. The present study aimed at investigating the presence of A. marginale in dairy cattle of Luz de América commune, province of Santo Domingo de los Tsachilas. Two PCRs were used to amplify specific regions of the Rickettsia for its molecular identification. Results At first, 151 blood samples were tested: msp5 specific gene of A. marginale was identified in 130 samples, meaning 86.1% of them were infected by the rickettsia. Two positive samples were further randomly selected to confirm the presence of A. marginale through amplification, cloning and sequencing of the conserved region of gene 16S rRNA. The analysis of sequences obtained through cloning revealed a 100% identity between both samples and those registered in GenBank for A. marginale. Conclusion This is the first report and molecular identification of A. marginale in the bovine population of Ecuador and its prevalence was high at the level of farms and animals. These results demonstrate the importance of proceeding to evaluate and characterize bovine Anaplasmosis in Ecuador in order to establish control measures and reduce their impact. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink