Small size fullerenol nanoparticles suppress lung metastasis of breast cancer cell by disrupting actin dynamics

Autor: Kui Chen, Li Zeng, Weihong Gu, Shan Li, Xue Bai, Shibo Xia, Ruihong Lei, Yanxia Qin, Gengmei Xing, Yanan Chang, Xinghua Dong, Sihan Ma, Jiaxin Zhang, Juan Li
Rok vydání: 2018
Předmět:
0301 basic medicine
Integrins
Lung Neoplasms
Pharmaceutical Science
Medicine (miscellaneous)
Apoptosis
02 engineering and technology
Applied Microbiology and Biotechnology
Metastasis
Cell Movement
Young’s modulus
Filopodia
biology
Chemistry
021001 nanoscience & nanotechnology
Cell biology
Actin Cytoskeleton
lcsh:R855-855.5
Molecular Medicine
Female
Fullerenes
0210 nano-technology
lcsh:Medical technology
Fullerenol nanoparticles
Cell Survival
lcsh:Biotechnology
Integrin
Biomedical Engineering
Motility
Mice
Nude
Bioengineering
Antineoplastic Agents
Breast Neoplasms
macromolecular substances
03 medical and health sciences
lcsh:TP248.13-248.65
Cell Line
Tumor
Elastic Modulus
medicine
Cell Adhesion
Animals
Humans
Actin
Research
Cancer
medicine.disease
Actin cytoskeleton
Actins
030104 developmental biology
Cancer cell
biology.protein
Nanoparticles
Actin dynamics
Zdroj: Journal of Nanobiotechnology
Journal of Nanobiotechnology, Vol 16, Iss 1, Pp 1-14 (2018)
ISSN: 1477-3155
Popis: Background Tumor metastasis is the primary cause of mortality in cancer patients. Migratory breast cancer cells in lymphatic and blood vessels seek new sites and form metastatic colonies in the lung and bone, and then these cancer cells often wreak considerable havoc. With advances in nanotechnology, nanomaterials and nanotechnologies are widely applied in tumor therapy. In this paper, small size fullerenol nanoparticles, which are separated by isoelectric focusing electrophoresis (IFE) for discrepancy of isoelectric point (pI), are used in the study of tumor metastasis. Results In this study, the commendable inhibition of tumor metastasis was uncovered by intravenous injection of purified fullerenol fraction with special surface charge and functional groups, which was separated by IFE for discrepancy of pI. By investigating the actin dynamics in several cancer cell lines, we found these small size fullerenol nanoparticles disturbed actin dynamics. Young’s modulus detection and cell migration assays revealed that fullerenol lowered stiffness and restrained migration of breast cancer cells. Filopodia, the main supporting structures of actin bundles, are important for cell motility and adhesion. Scanning electron microscopy showed that fullerenol reduced the number and length of filopodia. Simultaneously, the inhibition of integrin to form clusters on filopodias, which was likely induced by reorganizing of actin cytoskeleton, impacted cancer cell adhesion and motility. Conclusions With intravenous injection of these fullerenol nanoparticles, tumor metastasis is well inhibited in vivo. The underlying mechanism most likely to be attributed to the effect of fullerenol nanoparticles on disturbing actin dynamics. With the disordered actin fiber, cell function is varied, including decreased cell stiffness, reduced filopodia formation, and inactivated integrin. Electronic supplementary material The online version of this article (10.1186/s12951-018-0380-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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