Identification of CD244-expressing myeloid-derived suppressor cells in patients with active tuberculosis
| Autor: | Bingfen Yang, Zhai Fei, Xinjing Wang, Jing Jiang, Xiaoxing Cheng |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Immunology CD33 Population Nitric Oxide Synthase Type II Cell Count Adaptive Immunity CD8-Positive T-Lymphocytes Biology CD19 Immune tolerance Immune system Antigens CD Signaling Lymphocytic Activation Molecule Family Immune Tolerance Humans Immunology and Allergy Receptors Immunologic education Tuberculosis Pulmonary Cells Cultured Myeloid Progenitor Cells education.field_of_study HLA-DR Antigens Acquired immune system Molecular biology Immunity Innate Disease Progression biology.protein Myeloid-derived Suppressor Cell Female Biomarkers CD8 |
| Zdroj: | Immunology Letters. 158:66-72 |
| ISSN: | 0165-2478 |
| DOI: | 10.1016/j.imlet.2013.12.003 |
| Popis: | Development of active TB is accompanied by immune suppression and the underlining mechanisms have been explored extensively in recent years. MDSCs are a heterogeneous group of immature and progenitor myeloid cells with strong immunosuppressive ability for both natural and adaptive immunity. In our analysis of CD244 (2B4)-expressing cells in PBMCs from patients with active TB, a CD3(-)CD244(high) subpopulation was identified. A match of cell population in flow cytometry showed that nearly all CD3(-)CD244(high) cells were CD3(-)HLA-DR(-)CD11b(int)CD33(+) cells. The CD3(-)CD244(high) cell population has phenotypes of CD3(-)CD19(-)CD56(-)CD15(-)CD66b(-)CD33(+)CD11b(+)CD14(-)HLA-DR(neg/low), which was consistent with MDSCs in humans as previously reported. Patients with active TB had higher frequencies of CD3(-)CD244(high) cells as compared with healthy controls. The CD3(-)CD244(high) cell population had high levels of NOS2 expression and was negatively correlated with activation and effective molecule production of CD4(+) and CD8(+) T cells. In conclusion, CD3(-)CD244(high) cells had phenotypes of MDSCs and CD244 might be used as a marker for human CD3(-)HLA-DR(-)CD11b(int)CD33(+) MDSCs. |
| Databáze: | OpenAIRE |
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