Elevated immunoglobulin G antibodies to the proline-rich amino-terminal region of Epstein-Barr virus nuclear antigen-2 in sera from patients with systemic connective tissue diseases and from a subgroup of Sjögren's syndrome patients with pulmonary involvements
| Autor: | M Okawa-Takatsuji, S Aotsuka, Shuichi Kusano, Kazuo Yanagi, S Sato, Hiroyuki Eda, Ryo Kitamura, M Yamazaki |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Systemic disease Epstein-Barr Virus Infections viruses Immunology Enzyme-Linked Immunosorbent Assay medicine.disease_cause Antibodies Viral Immunoglobulin G Statistics Nonparametric Arthritis Rheumatoid Viral Proteins Antigen immune system diseases hemic and lymphatic diseases Clinical Studies Immunology and Allergy Medicine Humans Lupus Erythematosus Systemic Connective Tissue Diseases Lung Autoimmune disease biology business.industry medicine.disease Connective tissue disease Epstein–Barr virus Virus Latency Sjogren's Syndrome Epstein-Barr Virus Nuclear Antigens Case-Control Studies biology.protein Epstein–Barr virus nuclear antigen 2 Antibody business |
| Zdroj: | Clinical and experimental immunology. 139(3) |
| ISSN: | 0009-9104 |
| Popis: | SummaryAssociations of Epstein–Barr virus (EBV) and autoimmune diseases have been hypothesized. We have analysed IgG antibodies to EBV nuclear antigen (EBNA)-2 in sera from Japanese patients with autoimmune systemic connective tissue diseases (CTD), exemplified by systemic lupus erythematosus (SLE), primary Sjögren's syndrome (SS), rheumatoid arthritis (RA), systemic sclerosis (SSc) and secondary SS (classical CTDs complicated with SS). An enzyme-linked immunosorbent assay (ELISA) which uses glutathione-S-transferase polypeptides fused to EBV nuclear antigen (EBNA)-2 and EBNA-1 was developed. Ratios of IgG antibody reactivity to whole IgG concentrations of sera were calculated to normalize EBNA-2 and EBNA-1 antibody levels to the hypergammaglobulinaemia that occurs in CTD. The ELISA optical density OD450 readings of IgG antibodies to both the amino-terminal aa 1–116 of EBNA-2 and carboxyl-terminal aa 451–641 of EBNA-1 were elevated significantly in patients with SLE, primary SS, RA, SSc and secondary SS when compared to EBNA-1. The OD readings were divided by serum IgG concentrations to normalize for the hypergammaglobulinaemia. The specific levels of IgG antibodies to the amino-terminal region of EBNA-2 were elevated in patients with SLE, primary SS or RA, as well as those with secondary SS complicated with SLE or RA. The EBNA-2 amino-terminal region contains a polyproline tract and a proline-rich sequence and has considerable amino acid sequence homology with many cellular proline-rich proteins. High ratios of EBNA-2 aa 1–116 to EBNA-1 aa 451–641 IgG antibody levels which probably suggest reactivation of EBV latent infection were associated significantly with pulmonary involvement in SS patients. These results are consistent with the hypothesis that the sequence similarity between the amino-terminal region of EBNA-2 and proline-rich cellular proteins is associated with pathogenesis in a subpopulation of CTD patients, possibly by the molecular mimicry–epitope shift mechanism. |
| Databáze: | OpenAIRE |
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