Increased expression of prion protein gene is accompanied by demethylation of CpG sites in a mouse embryonal carcinoma cell line, P19C6
| Autor: | Eiko Matsuo, Wuyun Dalai, Natsumi Takeyama, Keiichi Saeki, Junichi Kawano |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Transcription Genetic Prions Cellular differentiation animal diseases Biology Prion Proteins PRNP Cell Line Epigenesis Genetic 03 medical and health sciences Mice Epigenetics of physical exercise Virology mental disorders Animals Epigenetics prion protein gene neuronal differentiation Regulation of gene expression Neurons General Veterinary epigenetics Cell Differentiation Methylation DNA Methylation Note Molecular biology nervous system diseases 030104 developmental biology CpG site Gene Expression Regulation DNA methylation CpG methylation CpG Islands |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | Elucidation of the processes regulating the prion protein gene (Prnp) is an important key to understanding the development of prion disorders. In this study, we explored the involvement of DNA methylation in Prnp transcriptional regulation during neuronal differentiation of embryonic carcinoma P19C6 cells. When P19C6 cells were differentiated into neuronal cells, the expression of Prnp was markedly increased, while CpG methylation was significantly demethylated at the nucleotide region between -599 and -238 from the transcription start site. In addition, when P19C6 cells were applied in a DNA methyltransferase inhibitor, RG108, Prnp transcripts were also significantly increased in relation to the decreased methylation statuses. These findings helped to elucidate the DNA methylation-mediated regulation of Prnp expression during neuronal differentiation. |
| Databáze: | OpenAIRE |
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