Characterization of heat exposure‐associated escape behaviors and HSP gene expression in bed bugs ( Cimex lectularius L.)
| Autor: | Ameya D. Gondhalekar, Michael E. Scharf, Aaron R. Ashbrook, Jeffrey L. Feder, Gary W. Bennett |
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| Rok vydání: | 2021 |
| Předmět: |
Bedbugs
Stress recovery education.field_of_study Behavioral experiment Veterinary medicine Hot Temperature biology Population Poultry house Gene Expression Ectoparasitic Infestations General Medicine biology.organism_classification Bed bug Insect Science Heat shock protein Gene expression Animals education Cimex lectularius Agronomy and Crop Science Heat-Shock Proteins |
| Zdroj: | Pest Management Science. 78:205-216 |
| ISSN: | 1526-4998 1526-498X |
| DOI: | 10.1002/ps.6620 |
| Popis: | Background Heat can be effective for bed bug elimination. However, in some cases bed bugs survive heat treatments. The objectives of this study were to determine the behavioral responses of bed bugs to rising harborage temperatures (23.0-49.0 °C) and identify which heat shock protein (HSP) genes are expressed after heat exposure. First, a custom-made copper arena and harborage were used to determine the escape behaviors of six bed bug populations. Next, HSP gene expression responses of select populations were determined after heat exposure using real time quantitative polymerase chain reaction (RT-qPCR). Results Analysis of the 25 min behavioral experiment data found that harborage top temperatures associated with 25%, 50% and 75% probabilities of bed bugs to flee the harborage did not differ significantly between populations. Also, the percentage of insects that escaped from heated areas and survived (4.0-12.0%) was not different between populations. However, when specific temperatures at which successful escapes occurred were statistically compared, the Poultry House population was found to flee the harborage at statistically higher temperatures (43.6 ± 0.5 °C) than others (40.5 ± 0.6-42.0 ± 0.7 °C). The RT-qPCR experiments revealed that the HSP70.1, HSP70.3, and Putative Small HSP genes were significantly up-regulated 15 min, 2, and 4 h post-heat exposure and decreased back to baseline levels by 24 h. Conclusions This study shows that when harborage top temperatures approach 40.0-43.0 °C, bed bugs will disperse in search for cooler areas. This work implicates the HSP70.1, HSP70.3, and Putative Small HSP genes in heat induced stress recovery of bed bugs. |
| Databáze: | OpenAIRE |
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