Long non-coding RNA musculin antisense RNA 1 promotes proliferation and suppresses apoptosis in osteoarthritic chondrocytes via the microRNA-369-3p/Janus kinase-2/ signal transducers and activators of transcription 3 axis
| Autor: | Zhenyu Tang, Zongming Gong, Xiaoliang Sun |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
STAT3 Transcription Factor Cell Survival Down-Regulation Apoptosis Bioengineering Applied Microbiology and Biotechnology stat3 Chondrocytes microrna-369-3p Humans Cells Cultured Cell Proliferation jak2 General Medicine Janus Kinase 2 Middle Aged musculin antisense rna 1 MicroRNAs osteoarthritis Female RNA Long Noncoding TP248.13-248.65 Research Article Research Paper Signal Transduction Biotechnology |
| Zdroj: | Bioengineered, Vol 13, Iss 1, Pp 1554-1564 (2022) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| Popis: | Increasing evidence indicates that long non-coding RNAs (lncRNAs) play critical roles in osteoarthritis (OA). The present study aimed to investigate the underlying molecular mechanism of lncRNA musculin antisense RNA 1 (MSC-AS1) in OA. RT-qPCR was used to detect MSC-AS1 levels in cartilage tissues from patients with OA. The effects of MSC-AS1 knockdown on the viability and apoptosis in OA were evaluated via CCK-8 and TUNEL assays. The StarBase database was used to predict the binding sites between microRNA (miR)-369-3p and MSC-AS1 or JAK2, which were confirmed via the dual-luciferase reporter assay. The results demonstrated that MSC-AS1 expression was downregulated in OA. Functional analysis indicated that the addition of MSC-AS1 promoted viability and inhibited inflammation and the apoptosis of chondrocytes. In addition, MSC-AS1 regulated the survival of OA chondrocytes by sponging miR-369-3p. JAK2 was confirmed as a direct target of miR-369-3p, and MSC-AS1 regulated JAK2/STAT3 signaling via miR-369-3p in OA chondrocytes. Taken together, our results suggest that MSC-AS1 may regulate the miR-369-3p/JAK2/STAT3 signaling pathway to accelerate the viability, and inhibit inflammation and cell apoptosis in OA chondrocytes. |
| Databáze: | OpenAIRE |
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