High levels of transforming growth factor beta 1 in patients with colorectal cancer: Association with disease progression
| Autor: | Sumio Kawata, Yasuharu Imai, Yuji Matsuzawa, Yasuhiro Shirai, Hirofumi Tsushima, Nobuyuki Ito, Hiromi Shimomukai, Shinichi Kiso, Yasuo Matsuda, Shinji Tamura, Yasuharu Nomura |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Cytoplasm medicine.medical_specialty Colorectal cancer Rectum Enzyme-Linked Immunosorbent Assay Adenocarcinoma Transforming Growth Factor beta Internal medicine Blood plasma Biomarkers Tumor medicine Humans RNA Messenger Aged Neoplasm Staging Aged 80 and over Messenger RNA Hepatology biology business.industry Gastroenterology Transforming growth factor beta Middle Aged Blotting Northern medicine.disease Immunohistochemistry Endocrinology medicine.anatomical_structure Tumor progression Disease Progression Cancer research biology.protein Female Colorectal Neoplasms business Transforming growth factor |
| Zdroj: | Gastroenterology. 110:375-382 |
| ISSN: | 0016-5085 |
| DOI: | 10.1053/gast.1996.v110.pm8566583 |
| Popis: | Contribution of transforming growth factor beta 1 (TGF-beta 1) to tumor progression has been suggested. However, little is known about the role of TGF-beta 1 in colorectal cancer. Plasma TGF-beta 1 levels and its expression were analyzed in patients with colorectal cancer.Plasma TGF-beta 1 levels were measured in 22 patients with colorectal cancer using a TGF-beta 1 enzyme-linked immunosorbent assay. Expression of TGF-beta 1 messenger RNA and immunohistochemical distribution of the protein in colorectal cancer tissues were examined.Plasma TGF-beta 1 levels in patients with colorectal cancer (14.8 +/- 8.4 ng/mL) were significantly higher than in normal controls (1.9 +/- 1.4; n = 22) (P0.001). After curative surgical resection, plasma TGF-beta 1 levels decreased in examined patients from 11.9 +/- 6.7 to 3.8 +/- 1.2 ng/mL (P0.01). TGF-beta 1 messenger RNA was about 2 1/2 times more abundant in colorectal cancer tissues than in control (P0.01). TGF-beta 1 was detected in the cytoplasm of colorectal cancer cells immunohistochemically. Both TGF-beta 1 messenger RNA expression in colorectal adenocarcinoma tissues and its plasma levels were associated with tumor stage of Dukes' classification (P0.05).These results suggest that plasma TGF-beta 1 levels may reflect overexpression of the gene in colon cancer tissues and are associated with disease progression. |
| Databáze: | OpenAIRE |
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