Experimental Meningococcal Sepsis in Congenic Transgenic Mice Expressing Human Transferrin
| Autor: | Corinne Ruckly, Muhamed-Kheir Taha, Eva Hong, Marek Szatanik, Dario Giorgini, Katarzyna Jopek, Morgan Ledroit, Marie-Anne Nicola, Ala-Eddine Deghmane |
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| Přispěvatelé: | Infections Bactériennes Invasives, Institut Pasteur [Paris], Imagerie Dynamique (Plate-Forme) (PFID), The work was supported exclusively by the Institut Pasteur with no external funding., Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Bacterial Diseases
Viral Diseases Critical Care and Emergency Medicine Mouse Microarrays Colony Count Microbial Gene Expression lcsh:Medicine Neisseria meningitidis medicine.disease_cause MESH: Meningococcal Infections Mice Molecular Cell Biology MESH: Animals lcsh:Science MESH: Sepsis Animal Management chemistry.chemical_classification Inhalation Exposure Mice Inbred BALB C 0303 health sciences MESH: Cytokines Multidisciplinary Transferrin Acute-phase protein Brain Agriculture Animal Models MESH: Gene Expression Regulation Bacterial Pathogens 3. Good health Infectious Diseases Influenza A virus MESH: Survival Analysis Medicine Cytokines MESH: Inhalation Exposure Transgenic Animals Injections Intraperitoneal MESH: Transferrin MESH: Injections Intraperitoneal Research Article MESH: Mice Transgenic MESH: Influenza A virus Congenic MESH: Mice Inbred BALB C Mice Transgenic Biology Meningococcal disease Microbiology MESH: Neisseria meningitidis Sepsis Mice Congenic 03 medical and health sciences MESH: Brain Model Organisms Immune system MESH: Mice Congenic medicine Animals Humans MESH: Mice MESH: Colony Count Microbial 030304 developmental biology Innate immune system MESH: Humans 030306 microbiology lcsh:R Computational Biology medicine.disease Survival Analysis [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Influenza Meningococcal Infections Gene Expression Regulation chemistry Immunology Meningoccal Septicemia lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2011, 6 (7), pp.e22210. ⟨10.1371/journal.pone.0022210⟩ PLoS ONE, Vol 6, Iss 7, p e22210 (2011) PLoS ONE, 2011, 6 (7), pp.e22210. ⟨10.1371/journal.pone.0022210⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0022210⟩ |
| Popis: | International audience; Severe meningococcal sepsis is still of high morbidity and mortality. Its management may be improved by an experimental model allowing better understanding of its pathophysiology. We developed an animal model of meningococcal sepsis in transgenic BALB/c mice expressing human transferrin. We studied experimental meningococcal sepsis in congenic transgenic BALB/c mice expressing human transferrin by transcriptional profiling using microarray analysis of blood and brain samples. Genes encoding acute phase proteins, chemokines and cytokines constituted the largest strongly regulated groups. Dynamic bioluminescence imaging further showed high blood bacterial loads that were further enhanced after a primary viral infection by influenza A virus. Moreover, IL-1 receptor-associated kinase-3 (IRAK-3) was induced in infected mice. IRAK-3 is a negative regulator of Toll-dependant signaling and its induction may impair innate immunity and hence result in an immunocompromised state allowing bacterial survival and systemic spread during sepsis. This new approach should enable detailed analysis of the pathophysiology of meningococcal sepsis and its relationships with flu infection. |
| Databáze: | OpenAIRE |
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