Do rat kidney cortex microsomes possess the enzymatic machinery to desaturate and chain elongate fatty acyl-CoA derivatives?
| Autor: | Lynda Cook, Peter Osei, Dominick L. Cinti, Mahmoud N. Nagi, Sanoj K. Suneja |
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| Rok vydání: | 1991 |
| Předmět: |
Fatty Acid Desaturases
Male Kidney Cortex Renal cortex Biology Reductase Biochemistry Substrate Specificity chemistry.chemical_compound Acyl-CoA Microsomes medicine Animals Enoyl-CoA Hydratase chemistry.chemical_classification Organic Chemistry Fatty acid Rats Inbred Strains Cell Biology Rats Alcohol Oxidoreductases Kinetics medicine.anatomical_structure Enzyme chemistry Microsome lipids (amino acids peptides and proteins) Arachidonic acid Acyl Coenzyme A Eicosanoid Production |
| Zdroj: | Lipids. 26:359-363 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/bf02537199 |
| Popis: | Rat kidney cortex microsomal preparations were unable to catalyze delta 9, delta 6 and delta 5 desaturation of stearoyl-coenzyme A (CoA), linoleoyl-CoA and dihomo-gamma-linolenoyl-CoA, respectively. The kidney cortex microsomal fraction, however, did catalyze the malonyl-CoA dependent fatty acyl-CoA elongation. The biochemical properties of palmitoyl-CoA elongation were studied as a function of protein concentration, time, reduced nicotinamide adenine dinucleotide phosphate (NADPH), malonyl-CoA and substrate concentrations; of the substrates investigated, delta 6,9,12-18:3 was the most active. Unlike what was observed in the hepatic system, a high-carbohydrate, fat-free diet did not induce kidney fatty acid chain elongation. All intermediate kidney cortex microsomal reactions, i.e., beta-ketoacyl-CoA reductase, beta-hydroxyacyl-CoA dehydrase and trans-2-enoyl-CoA reductase activities, were significantly higher (greater than one order of magnitude) than the condensing enzyme activity, suggesting that the rate-limiting step in total elongation is the initial condensation reaction. Contrary to other reports, the results suggest that the kidney cannot synthesize arachidonic acid needed for eicosanoid production. |
| Databáze: | OpenAIRE |
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