Polyclonal emergence and importation of community-acquired methicillin-resistant Staphylococcus aureus strains harbouring Panton-Valentine leucocidin genes in Belgium

Autor: Anne Vergison, Marc Struelens, G. Huysmans, Youri Glupczynski, Marie Hallin, X. Malaviolle, H. De Beenhouwer, Olivier Denis, M. G. Garrino, Ariane Deplano
Rok vydání: 2005
Předmět:
Adult
DNA
Bacterial
Male
Microbiology (medical)
Staphylococcus aureus
Adolescent
Virulence Factors
Bacterial Toxins
Exotoxins
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Microbiology
Bacterial Proteins
Belgium
Leukocidins
Drug Resistance
Bacterial
medicine
Humans
Pharmacology (medical)
Typing
Child
Aged
Pharmacology
Molecular Epidemiology
Molecular epidemiology
SCCmec
Infant
Kanamycin
Middle Aged
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Virology
Anti-Bacterial Agents
Community-Acquired Infections
Infectious Diseases
Child
Preschool
bacteria
Multilocus sequence typing
Female
Methicillin Resistance
Panton–Valentine leukocidin
Polymorphism
Restriction Fragment Length
medicine.drug
Zdroj: Journal of Antimicrobial Chemotherapy. 56:1103-1106
ISSN: 1460-2091
0305-7453
Popis: Objectives: Worldwide spread of a limited number of Panton-Valentine leucocidin (PVL) -producing methicillin-resistant Staphylococcus aureus (MRSA) clones has been reported in various communities. The objective of this study was to describe the molecular characteristics of the first PVL-positive MRSA strains isolated in Belgium. Methods: Clinical MRSA isolates (n = 41) collected from 2002 to 2004 from Belgian patients were investigated for the PVL gene by PCR. PVL-positive isolates were genotyped by PFGE, staphylococcal cassette chromosome mec (SCCmec) typing, spa sequence typing, accessory gene regulator (agr) polymorphism and multi-locus sequence typing (MLST). Susceptibility to 14 antimicrobials was determined by the disc diffusion method. Genes encoding resistance to tetracyclines, aminoglycosides and macrolide-lincosamide-streptogramin were determined by PCR. Results: Sixteen isolates carried lukS-lukF genes that encode the PVL toxin. All but one isolate were community-acquired. Three patients reported recent travel to North Africa and South America. They were associated with skin or soft tissue infections, bacteraemia and peritonitis. By molecular typing, they belonged to five genotypes: ST80-SCCmec IV, ST8-SCCmec IV, ST30-SCCmec IV, ST153-SCCmec IV and ST88-SCCmec IV. They belonged to the agr type 3 except for ST8 strains, which showed agr type 1. All isolates were susceptible to fluoroquinolones. Approximately, half of them were resistant to tetracycline, fusidic acid and kanamycin. Tetracycline-resistant strains harboured the tet(K) gene and resistance to kanamycin was associated with the aph(3')-IIIa gene. The single erythromycin-resistant isolate harboured msr(A/B) genes conferring the M resistance phenotype. Conclusions: These results indicate the recent emergence and sporadic importation into Belgium of PVL-positive community-associated MRSA strains belonging to five distinct clones.
Databáze: OpenAIRE
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