Inhibitive Effects of Huashi Pill on Formation of Renal Stones by Modulating Urine Biochemical Indexes and Osteopontin in Renal Stone Rat Models
| Autor: | Manling Fu, Liu Minyong, Yang Ailing, Guo Huimeng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty China Bilirubin Urine 030204 cardiovascular system & hematology Kidney Blood Urea Nitrogen Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Kidney Calculi 0302 clinical medicine Internal medicine medicine Animals Medicine Chinese Traditional Blood urea nitrogen Calcium metabolism Creatinine Calcium Oxalate Animal Study General Medicine Liquid Crystals Rats Uric Acid Disease Models Animal Endocrinology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Uric acid Calcium Osteopontin Calreticulin TBIL Drugs Chinese Herbal |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 1234-1010 |
| Popis: | BACKGROUND Renal stones are the accumulated or deposited crystals that form and appear in supersaturated urine. This study aimed to the investigate the therapeutic effects of Huashi Pill on clearance of renal stones. MATERIAL AND METHODS Sprague Dawley rats were divided into normal control, positive control, low-dosage Huashi Pill, medium-dosage Huashi Pill, and high-dosage Huashi Pill groups. A renal rat model was established by using ethylene glycol, ammonium chloride, and calcium gluconate. The urinary pH, urine protein, and uric acid levels, as well as the calcium, magnesium, and phosphorus levels were examined. The blood urea nitrogen (BUN) and creatinine (Cr) levels were also evaluated. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TBIL) levels were evaluated. Crystal formation and calcium deposits were examined using hematoxylin and eosin (H and E) staining and von Kossa staining, respectively. Osteopontin (OPN) expression was evaluated with quantitative real-time polymerase chain reaction assay and immunohistochemical assay. RESULTS A renal stone rat model was successfully established. Huashi Pill significantly improved water and food intake and enhanced pH value of urine (P |
| Databáze: | OpenAIRE |
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