Transgene and mitochondrial DNA are indicators of efficient composting of transgenic pig carcasses
| Autor: | John P. Phillips, Serguei P. Golovan, Roy G. Meidinger, Ivan P. O'Halloran, Cecil W. Forsberg, R. R. Hacker, Dave Murray, Ming Z. Fan |
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| Rok vydání: | 2007 |
| Předmět: |
Genetic Markers
Litter (animal) Mitochondrial DNA Veterinary medicine Environmental Engineering Swine Transgene Bioengineering engineering.material Biology DNA Mitochondrial Polymerase Chain Reaction complex mixtures law.invention Animals Genetically Modified Soil law Animals Transgenes Waste Management and Disposal Humic Substances Polymerase chain reaction 6-Phytase Renewable Energy Sustainability and the Environment business.industry Compost fungi Genetic transfer DNA General Medicine Molar Biotechnology Genetically modified organism engineering Phytase business |
| Zdroj: | Bioresource Technology. 98:1795-1804 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2006.06.029 |
| Popis: | Composting is an environmentally sound method for the disposal of on-farm livestock mortalities that generates material suitable for use as fertilizer; however, this method is not generally permitted for disposal of transgenic livestock mortalities during the research and development phase. This study has explored the application of the polymerase chain reaction (PCR) as a method for assessing the persistence of transgene and mitochondrial DNA markers during the composting of euthanized transgenic pig. There was at least a 10(7) fold reduction of genetic material to a level that not either transgene or mitochondrion markers were detectable. At the end of the composting period, only bone fragments that were completely demineralised and chalky were detected. Chemically the compost was similar to that from pig litter and poultry mortalities, except the copper content was lower. Based on these data, composting appears to be an appropriate method for the disposal of transgenic animals. |
| Databáze: | OpenAIRE |
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