Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips
| Autor: | Christian Mazza, Jan Roelof van der Meer, Xavier Richard, Clémence Roggo, Harald van Lintel, Cristian Picioreanu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microfluidics 030106 microbiology Biosensing Techniques Biology medicine.disease_cause Microbiology 03 medical and health sciences Escherichia coli Serine medicine Electrochemical gradient Ecology Evolution Behavior and Systematics Aspartic Acid Reporter gene Herbicides Chemotaxis Cupriavidus 2 4-Dichlorophenoxyacetic Acid/analysis Aspartic Acid/analysis Biosensing Techniques/methods Chemotaxis/physiology Cupriavidus/physiology Environmental Pollutants/analysis Escherichia coli/physiology Herbicides/analysis Microfluidics/methods Serine/chemistry 030104 developmental biology Biochemistry Environmental Pollutants Steady state (chemistry) Bioreporter 2 4-Dichlorophenoxyacetic Acid Biosensor |
| Zdroj: | Environmental microbiology, vol. 20, no. 1, pp. 241-258 Environmental Microbiology, 20(1) |
| ISSN: | 1462-2912 |
| Popis: | Whole-cell bacterial bioreporters are proposed as alternatives to chemical analysis of, for example, pollutants in environmental compartments. Commonly based on reporter gene induction, bioreporters produce a detectable signal within 30 min to a few hours after exposure to the chemical target, which is impractical for applications aiming at a fast response. In an attempt to attain faster readout but maintain flexibility of chemical targeting, we explored the concept for quantitative chemical sensing by bacterial chemotaxis. Chemotaxis was quantified from enrichment of cells across a 600 µm-wide chemical gradient stabilized by parallel flow in a microfluidic chip, further supported by transport and chemotaxis steady state and kinetic modelling. As proof-of-concept, we quantified Escherichia coli chemotaxis towards serine, aspartate and methylaspartate as a function of attractant concentration and exposure time. E. coli chemotaxis enrichment increased sharply between 0 and 10 µM serine, before saturating at 100 µM. The chemotaxis accumulation rate was maximal at 10 µM serine, leading to observable cell enrichment within 5 min. The potential application for biosensing of environmental toxicants was investigated by quantifying chemotaxis of Cupriavidus pinatubonensis JMP134 towards the herbicide 2,4-dichlorophenoxyacetate. Our results show that bacterial chemotaxis can be quantified on a scale of minutes and may be used for developing faster bioreporter assays. |
| Databáze: | OpenAIRE |
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