Isolation and identification of methanethiol-utilizing bacterium CZ05 and its application in bio-trickling filter of biogas
| Autor: | Jiao Xu, Chao-zheng Zhang, Weijiang Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Environmental Engineering
Trickling filter Hydrogen sulfide Sewage Bioengineering Methanethiol chemistry.chemical_compound Bioreactors Biogas Hydrogen Sulfide Sulfhydryl Compounds Waste Management and Disposal Enzyme Assays Chromatography biology Waste management Renewable Energy Sustainability and the Environment business.industry General Medicine biology.organism_classification Filter (aquarium) Biodegradation Environmental chemistry Biofuels Fermentation Paenibacillus polymyxa business Paenibacillus Filtration Sludge |
| Zdroj: | Bioresource Technology. 150:338-343 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2013.10.024 |
| Popis: | A bacterium capable of methanethiol (MT) degradation was enriched and isolated by employing activated sewage sludge as the inoculum in a mineral medium containing MT. The isolate was identified as Paenibacillus polymyxa CZ05 through a Biolog test and 16S rDNA sequencing. This strain can utilize both organic and inorganic media and thrives at pH 4 to 9. The batch culture showed that the strain can degrade MT better in the No. 4 medium than in the No. 1 medium. A series-operating biotrickling filter with lava stone as the carrier was employed to test the application of P. polymyxa CZ05 in the removal of MT in simulated biogas. Long-term experiments showed that a high concentration of MT (60 ppm) was efficiently removed (99.5%) by the biotrickling filters at EBRT 30 s. The addition of hydrogen sulfide decreased the MT removal rate because the dissolved oxygen competed with MT. |
| Databáze: | OpenAIRE |
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