Modeling human retinoblastoma using embryonic stem cell-derived retinal organoids
| Autor: | Zi-Qi Hua, Zi-Bing Jin, Hui Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Science (General)
Cellular differentiation Cell Culture Techniques Biology Models Biological Retina General Biochemistry Genetics and Molecular Biology Pathogenesis chemistry.chemical_compound Q1-390 Protocol Organoid medicine Humans Cells Cultured Embryonic Stem Cells Cancer General Immunology and Microbiology Retinoblastoma General Neuroscience Stem Cells Retinal Cell Differentiation medicine.disease Embryonic stem cell Organoids chemistry Cell culture Cancer research Stem cell |
| Zdroj: | STAR Protocols, Vol 2, Iss 2, Pp 100444-(2021) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | Summary Retinoblastoma (Rb) is the most prevalent intraocular malignancy in early childhood. Traditional models are unable to accurately recapitulate the origin and development of human Rb. Here, we present a protocol to establish a novel human Rb organoid (hRBO) model derived from genetically engineered human embryonic stem cells (hESCs). This hRBO model exhibits properties highly consistent with human primary Rb and can be used effectively for dissecting the origination and pathogenesis of Rb as well as for screening of potential therapies. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020). Graphical abstract Highlights • Detailed protocol to generate hESCs with mutagenesis of the RB1 gene • Protocol to generate an in-dish human Rb organoid system from RB1 mutant hESCs • Steps for quality control and assessment of hRBO • Human Rb organoids (hRBO) recapitulate key features of human primary Rb Retinoblastoma (Rb) is the most prevalent intraocular malignancy in early childhood. Traditional models are unable to accurately recapitulate the origin and development of human Rb. Here, we present a protocol to establish a novel human Rb organoid (hRBO) model derived from genetically engineered human embryonic stem cells (hESCs). This hRBO model exhibits properties highly consistent with human primary Rb and can be used effectively for dissecting the origination and pathogenesis of Rb as well as for screening of potential therapies. |
| Databáze: | OpenAIRE |
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