Glomerular Pyrimidine Metabolism in Experimental Diabetic Nephropathy
| Autor: | B.H. Spargo, P. Cortes, Francis Dumler, K. K. Venkatachalam, J. Goldman, Nathan W. Levin |
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| Rok vydání: | 1980 |
| Předmět: |
Male
medicine.medical_specialty Kidney Cortex Time Factors Uracil Nucleotides DNA biosynthesis Kidney Glomerulus Basement Membrane Diabetes Mellitus Experimental Diabetic nephropathy In vivo Diabetes mellitus Internal medicine medicine Animals Orotic Acid Chemistry DNA General Medicine Metabolism medicine.disease Streptozotocin Rats Pyrimidines Endocrinology Nephrology Pyrimidine metabolism RNA Cardiology and Cardiovascular Medicine Uracil nucleotide medicine.drug |
| Zdroj: | Kidney and Blood Pressure Research. 3:79-87 |
| ISSN: | 1423-0143 1420-4096 |
| Popis: | Glomerular uracil nucleotide metabolism was studied in vivo in control and diabetic rats 48 h after the injection of streptozotocin. The animals were infused for 2 h with 3H-orotate and the fraction of infused dpm incorporated into glomerular uracil nucleotides and RNA/mg of glomerular DNA was calculated. Diabetic glomeruli showed an increase in RNA/DNA compared to controls (p < 0.05) and a greater incorporation of 3H-orotate into uracil nucleotides and RNA. These changes were reversed by insulin therapy. In separate experiments the renal cortical uracil nucleotide pool was expanded by feeding chow supplemented with 0.5% orotate to rats for 6 months. Normal animals fed orotate developed significant glomerular basement membrane thickening (p < 0.01) when compared to age-matched controls, which was morphologically indistinguishable from that of diabetics. Orotate feeding also produced further basement membrane thickening in diabetic rats. These results suggest that early diabetes is characterized by an increase in glomerular uracil nucleotides, and that chronic expansion of the uracil nucleotide pool is associated with glomerular basement membrane thickening. |
| Databáze: | OpenAIRE |
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