Malignant transformation of rat thyroid cells transfected with the human TSH receptor cDNA
| Autor: | Simon Aeschimann, Martina Broecker, Hugo Studer, Michael Derwahl, H. Schatz |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
endocrine system
medicine.medical_specialty endocrine system diseases medicine.medical_treatment Molecular Sequence Data Thyroid Gland Biophysics Gene Expression Mice Nude Thyrotropin Biology Transfection Thyroglobulin Biochemistry Cell Line Thyrotropin receptor Malignant transformation Mice Internal medicine Complementary DNA medicine Animals Humans RNA Messenger Receptor Molecular Biology Base Sequence Dose-Response Relationship Drug Contact inhibition Receptors Thyrotropin Neoplasms Experimental Cell Biology Iodides Molecular biology Rats Cell Transformation Neoplastic Endocrinology Cell culture hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochemical and Biophysical Research Communications. 183:220-226 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)91631-y |
| Popis: | Growth and function of well differentiated FRTL-5 thyroid cells depend on thyrotropin as its main regulatory hormone. We demonstrate here that stable transfection of FRTL-5 cells with the human thyrotropin receptor cDNA results in cellular transformation of these cells with altered cell shape and loss of contact inhibition. The transformed cells replicate in soft agar and form invasive tumors when cell suspensions are implanted onto nude mice. They have lost their thyrotropin dependent growth and their ability to concentrate iodide and synthesize thyroglobulin. But they still express the rat thyrotropin receptor mRNA and accumulate cAMP in response to thyrotropin stimulation. However, although the full length human thyrotropin receptor cDNA is integrated into their genome, transformed cells do not express the human thyrotropin receptor mRNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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