Biodegradable Mg-Cu alloy implants with antibacterial activity for the treatment of osteomyelitis: In vitro and in vivo evaluations
| Autor: | Kerong Dai, Ke Yang, Degang Yu, Xinhua Qu, Lina Liu, Lili Tan, Peng Wan, Zhenan Zhu, Zhengxiao Ouyang, Zhenyang Mao, Yang Li, Ling Ren, Zanjing Zhai, Qi Sun, Yongqiang Hao |
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| Rok vydání: | 2016 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Materials science Biocompatibility medicine.drug_class Antibiotics Biophysics Bioengineering 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Microbiology Biomaterials In vivo Absorbable Implants medicine Alloys Animals Magnesium Drug Implants Osteomyelitis technology industry and agriculture Biofilm Staphylococcal Infections equipment and supplies 021001 nanoscience & nanotechnology medicine.disease In vitro 0104 chemical sciences Anti-Bacterial Agents Treatment Outcome Mechanics of Materials Ceramics and Composites Rabbits 0210 nano-technology Antibacterial activity Staphylococcus Copper Biomedical engineering |
| Zdroj: | Biomaterials. 106 |
| ISSN: | 1878-5905 |
| Popis: | Treatment of chronic osteomyelitis (bone infection) remains a clinical challenge; in particular, it requires an implantable material with improved antibacterial activity. Here, we prepared biodegradable magnesium (Mg)-copper (Cu) alloys with different Cu contents (0.05, 0.1, and 0.25 wt%) and assessed their potential for treating methicillin-resistant Staphylococcus aureus-induced osteomyelitis. We evaluated the microstructures, mechanical properties, corrosion behavior, and ion release of the alloys in vitro, and their biocompatibility and antibacterial activity in vitro and in vivo. The antibacterial activity of the Mg-Cu alloys in vitro was demonstrated by microbiological counting assays, bacterial viability assays, biofilm formation observations, and the expression of biofilm, virulence, and antibiotic-resistance associated genes. The antibacterial activity of Mg-Cu alloys in vivo was confirmed by imaging examination, microbiological cultures, and histopathology. The biocompatibility of Mg-Cu alloys was confirmed by cell proliferation, vitality, and morphology assays in vitro and Cu(2+) or Mg(2+) ion assays, blood biochemical tests, and histological evaluation in vivo. The alloy containing 0.25 wt% Cu exhibited the highest antibacterial activity among the tested alloys, with favorable biocompatibility. Collectively, our results indicate the potential utility of Mg-Cu alloy implants with 0.25 wt% Cu in treating orthopedic infections. |
| Databáze: | OpenAIRE |
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