T cells in cardiac allograft vasculopathy are skewed to memory Th-1 cells in the presence of a distinct Th-2 population
| Autor: | N. de Jonge, F. H. J. Gmelig Meyling, R.A. de Weger, E. Siera-de Koning, J. van Kuik, M.E.I. Schipper, M.F.M. van Oosterhout, D.F. van Wichen, M. C. Hagemeijer |
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| Přispěvatelé: | Strategic Infection Biology, Dep Infectieziekten Immunologie |
| Rok vydání: | 2008 |
| Předmět: |
Neointima
Cellular immunity Pathology medicine.medical_specialty T-Lymphocytes Population CXCR3 Th2 Cells Humans Transplantation Homologous Immunology and Allergy Medicine Pharmacology (medical) education Transplantation education.field_of_study business.industry CD28 FOXP3 hemic and immune systems Th1 Cells International Circulatory system cardiovascular system Heart Transplantation business Immunologic Memory |
| Popis: | Cardiac allograft vasculopathy (CAV) in heart transplantation (HTx) patients remains the major complication for long-term survival, due to concentric neointima hyperplasia induced by infiltrating mononuclear cells (MNC). Previously, we showed that activated memory T-helper-1 (Th-1) cells are the major component of infiltrating MNC in coronary arteries with CAV. In this study, a more detailed characterization of the MNC in human coronary arteries with CAV (n = 5) was performed and compared to coronary arteries without CAV (n = 5), by investigating MNC markers (CD1a, DRC-1, CD3, CD20, CD27, CD28, CD56, CD68, CD69, FOXP3 and HLA-DR), cytokines (IL-1A, 2, 4, 10, 12B, IFN-gamma, and TGF-beta1), and chemokine receptors (CCR3, CCR4, CCR5, CCR7, CCR8, CXCR3 and CX3CR1) by immunohistochemical double-labeling and quantitative PCR on mRNA isolated from laser microdissected layers of coronary arteries. T cells in the neointima and adventitia of CAV were skewed toward an activated memory Th-1 phenotype, but in the presence of a distinct Th-2 population. FOXP3 positive T cells were not detected and production of most cytokines was low or absent, except for IFN-gamma, and TGF-beta. This typical composition of T-helper cells and especially production of IFN-gamma and TGF-beta may play an important role in the proliferative CAV reaction. |
| Databáze: | OpenAIRE |
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