Nicotinic Cholinergic Receptor Expression in the Human Nasal Mucosa
| Autor: | James C. Walker, C. Jane Keiger, L. Douglas Case, Martin Kendal-Reed, Kim R. Jones, Amelia F. Drake |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Respiratory Mucosa Nicotine Pathology medicine.medical_specialty Molecular Sequence Data Gene Expression Mucous membrane of nose Receptors Nicotinic Biology Epithelium 03 medical and health sciences Sex Factors 0302 clinical medicine Gene expression otorhinolaryngologic diseases medicine Humans RNA Messenger Sinusitis Child 030223 otorhinolaryngology Acetylcholine receptor Base Sequence Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Smoking General Medicine Middle Aged Molecular biology Trachea Reverse transcription polymerase chain reaction Nasal Mucosa medicine.anatomical_structure Nicotinic agonist Otorhinolaryngology 030220 oncology & carcinogenesis Chronic Disease Female Acetylcholine medicine.drug |
| Zdroj: | Annals of Otology, Rhinology & Laryngology. 112:77-84 |
| ISSN: | 1943-572X 0003-4894 |
| DOI: | 10.1177/000348940311200115 |
| Popis: | Twenty-four nasal mucosa specimens were obtained from the inferior or middle turbinates of 6 normal subjects and 18 patients with chronic sinusitis, inflammatory polyp formation, or sinus allergies. Reverse transcription-polymerase chain reaction analysis was used to identify the non-neuronal nicotinic cholinergic receptor (nAChR) subunits that were expressed in the nasal mucosa. Collectively, transcripts for alpha (alpha1, alpha2, alpha3, alpha4, alpha6, alpha7) and beta (beta2, beta3, beta4) nAChR subunit genes were detected in the respiratory mucosa. The alpha3, alpha7, and beta2 subunits were expressed in 92%, 88%, and 75% of the subjects, respectively. There was a high degree of interindividual variation in nAChR subunit gene expression among subjects. A significant univariate association was found between tissue type and beta4 expression and between gender and beta3 expression. These data suggest that cells in the nasal mucosa express the necessary messenger RNAs (mRNAs) for numerous nAChR combinations. Moreover, our identification of nAChR subunit mRNAs in the nasal mucosa extends the findings of other functional studies of nAChRs in nasal epithelial cells and implies that nicotine from tobacco products such as cigarette smoke and nicotine nasal spray may have direct cellular effects on nasal mucosa cells through activation of homogeneous or heterogeneous nAChRs. A significant number of patients receiving nicotine nasal spray have reported nasal irritation, and there are reports of transient irritation of the throat and trachea with the use of smoke-free nicotine cigarettes. These adverse respiratory effects may be due to activation of nAChRs in epithelial cells of the nose and trachea. |
| Databáze: | OpenAIRE |
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