Bacterial survival in whole blood depends on plasma sensitivity and resistance to neutrophil killing
| Autor: | Wendy M. Toyofuku, Dana L. Kyluik-Price, Mariam Taha, Mark D. Scott, Sandra Ramirez-Arcos, Dilini Kumaran |
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| Rok vydání: | 2019 |
| Předmět: |
Blood Platelets
Staphylococcus aureus Erythrocytes Neutrophils Immunology 030204 cardiovascular system & hematology Serratia liquefaciens medicine.disease_cause Microbiology Streptococcus agalactiae 03 medical and health sciences Plasma Staphylococcus capitis 0302 clinical medicine Staphylococcus epidermidis medicine Escherichia coli Leukocytes Immunology and Allergy Humans Serratia marcescens Whole blood Yersinia enterocolitica Microbial Viability biology Hematology biology.organism_classification Klebsiella pneumoniae Blood Preservation Bacteria 030215 immunology |
| Zdroj: | TransfusionREFERENCES. 59(12) |
| ISSN: | 1537-2995 |
| Popis: | BACKGROUND Whole blood (WB) is held at room temperature for not more than 24 hours before blood component manufacturing. The ability of several culture collection, skin-derived, and transfusion-related bacteria to survive in WB stored at 22 ± 2°C for 24 hours was investigated in this study. STUDY DESIGN AND METHODS Twenty-one bacteria of the species Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus capitis, Streptococcus agalactiae, Serratia liquefaciens, Serratia marcescens, Klebsiella pneumoniae, Escherichia coli, and Yersinia enterocolitica were inoculated into 7-mL aliquots of WB at a concentration of 500 colony-forming units (CFU)/mL. Spiked WB was stored aerobically at 22 ± 2°C, and bacterial viability and growth were monitored at 3, 8, and 24 hours during WB storage. Bacteria that showed decreased viability during WB incubation were further characterized for their sensitivity to plasma factors and neutrophil killing. RESULTS There were three different scenarios for bacterial behavior during the hold of WB at 22 ± 2°C. Five bacteria proliferated (p |
| Databáze: | OpenAIRE |
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