Cell culture studies on neurofibromatosis (von Recklinghausen). I. Comparative growth experiments with fibroblasts at high and low concentrations of fetal calf serum
| Autor: | Winfrid Krone, Sybille Zörlein, Richard Mao |
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| Rok vydání: | 1981 |
| Předmět: |
Adult
Fetal Proteins Male medicine.medical_specialty Neurofibromatosis 1 Cell division Adolescent Genotype Population Cell Cell Count Biology Internal medicine Genetics medicine Humans Neurofibromatosis education Genetics (clinical) Cells Cultured Fetal protein education.field_of_study Fetus Proteins Fibroblasts medicine.disease Molecular biology Culture Media Endocrinology medicine.anatomical_structure Cell culture Female Cell Division |
| Zdroj: | Human genetics. 58(2) |
| ISSN: | 0340-6717 |
| Popis: | The growth of strains of fibroblasts derived from patients with neurofibromatosis (NF) was compared with that of strains from appropriate controls in culture medium containing 1% or 15% fetal calf serum. The means of the ratios of final to initial cell numbers do not differ significantly between NF strains and control strains. Weakly significant differences are, however, obtained after conversion of the results to mean numbers of cell population doublings, the NF strains showing the higher numbers. The ratios of final to initial amounts of protein also differ significantly under both sets of growth conditions. High growth parameters occur significantly more frequently among our sample of 11 NF strains than among our sample of 13 control strains. The possibility of the expression of the NF genotype(s) on the level of the cultured fibroblast-like cells and the possible causes of the large ranges of inter- and intra-individual variations of the results are discussed. |
| Databáze: | OpenAIRE |
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