Microstructural composition, ion release, and bioactive potential of new premixed calcium silicate–based endodontic sealers indicated for warm vertical compaction technique

Autor: Julia Guerrero-Gironés, Carmen Llena, Leopoldo Forner, F.J. Rodríguez-Lozano, Adrián Lozano, Sergio López-García, María P. Pecci-Lloret, José Luis Sanz
Rok vydání: 2020
Předmět:
Zdroj: Clinical Oral Investigations. 25:1451-1462
ISSN: 1436-3771
1432-6981
Popis: The aim of this study was to evaluate the microstructural composition, ion release, cytocompatibility, and mineralization potential of Bio-C Sealer ION+ (BCI) and EndoSequence BC Sealer HiFlow (BCHiF), compared with AH Plus (AHP), in contact with human periodontal ligament cells (hPDLCs). The sealers’ ionic composition and release were assessed using energy-dispersive spectroscopy (EDS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. For the biological assays, hPDLCs were isolated from third molars, and sealer extracts were prepared (undiluted, 1:2, and 1:4 ratios). An MTT assay, wound-healing assay, and cell morphology and adhesion analysis were performed. Activity-related gene expression was determined using RT-qPCR, and mineralization potential was assessed using Alizarin Red staining (ARS). Statistical analyses were performed using one-way ANOVA and Tukey’s post hoc test (α
Databáze: OpenAIRE