TASK-Like Conductances Are Present within Hippocampal CA1 Stratum Oriens Interneuron Subpopulations
| Autor: | Douglas A. Bayliss, Brian W. Jeffries, Allison P. Berg, Chris J. McBain, Christine L. Torborg |
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| Rok vydání: | 2006 |
| Předmět: |
Patch-Clamp Techniques
Interneuron Glutamate decarboxylase Nerve Tissue Proteins In Vitro Techniques Biology Hippocampal formation Inhibitory postsynaptic potential Hippocampus behavioral disciplines and activities Mice Potassium Channels Tandem Pore Domain Interneurons medicine Animals Homomeric Patch clamp Glutamate Decarboxylase musculoskeletal neural and ocular physiology General Neuroscience Hydrogen-Ion Concentration Bupivacaine Immunohistochemistry Resting potential Potassium channel Isoenzymes Mice Inbred C57BL Parvalbumins medicine.anatomical_structure nervous system Somatostatin Brief Communications Neuroscience psychological phenomena and processes |
| Zdroj: | The Journal of Neuroscience. 26:7362-7367 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.1257-06.2006 |
| Popis: | TASK-1 (KCNK3) and TASK-3 (KCNK9) are members of the two-pore domain potassium channel family and form either homomeric or heteromeric open-rectifier (leak) channels. Recent evidence suggests that these channels contribute to the resting potential and input resistance in several neuron types, including hippocampal CA1 pyramidal cells. However, the evidence for TWIK-related acid-sensitive potassium (TASK)-like conductances in inhibitory interneurons is less clear, and mRNA expression has suggested that TASK channels are expressed in only a subpopulation of interneurons. Here we use immunocytochemistry to demonstrate prominent TASK-3 protein expression in both parvalbumin-positive- and a subpopulation of glutamic acid decarboxylase (GAD)67-positive interneurons. In addition, a TASK-like current (modulated by both pH and bupivacaine) was detected in 30–50% of CA1 stratum oriens interneurons of various morphological classes. In most neurons, basic shifts in pH had a larger effect on the TASK-like current than acidic, suggesting that the current is mediated by TASK-1/TASK-3 heterodimers. These data suggest that TASK-like conductances are more prevalent in inhibitory interneurons than previously supposed. |
| Databáze: | OpenAIRE |
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