The Initiation Knot is a Signaling Center Required for Molar Tooth Development
| Autor: | Jacqueline E. Moustakas-Verho, Minna Niittykoski, Laura Ahtiainen, Isabel Mogollón |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Molar
Cell division Confocal Cell Embryonic Development Apoptosis Cell Cycle Proteins Shh 03 medical and health sciences Wnt Mice 0302 clinical medicine stomatognathic system Cell Movement Embryonic morphogenesis medicine Gene silencing Animals Migration 030304 developmental biology Cell Proliferation 0303 health sciences Chemistry Wnt signaling pathway Tooth Germ Epithelial Cells Signaling center Cell cycle Cell biology DNA-Binding Proteins stomatognathic diseases medicine.anatomical_structure Odontogenesis Tooth 030217 neurology & neurosurgery Research Article Signal Transduction |
| Zdroj: | Development (Cambridge, England) article-version (VoR) Version of Record |
| DOI: | 10.1101/2020.04.09.033589 |
| Popis: | Signaling centers, or organizers, regulate many aspects of embryonic morphogenesis. In the mammalian molar tooth, reiterative signaling in specialized centers called enamel knots (EKs) determines tooth patterning. Preceding the primary EK, transient epithelial thickening appears, the significance of which remains debated. Using tissue confocal fluorescence imaging with laser ablation experiments, we show that this transient thickening is an earlier signaling center, the molar initiation knot (IK), that is required for the progression of tooth development. IK cell dynamics demonstrate the hallmarks of a signaling center: cell cycle exit, condensation and eventual silencing through apoptosis. IK initiation and maturation are defined by the juxtaposition of cells with high Wnt activity to Shh-expressing non-proliferating cells, the combination of which drives the growth of the tooth bud, leading to the formation of the primary EK as an independent cell cluster. Overall, the whole development of the tooth, from initiation to patterning, is driven by the iterative use of signaling centers. Summary: During tooth morphogenesis, transient thickening of the epithelium in the diastema anterior to the first developing molar is an early signaling center, the molar initiation knot (IK), which is required for the progression of mammalian molar tooth development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|