Corrigendum: Comparative proteomes change and possible role in different pathways of microRNA-21a-5p in a mouse model of spinal cord injury
| Autor: | Weiming Gong, Almaghalsa-Ziad Mohammed, Hong-liang Song, Tanghong Jia, Hong-Xia Du, Bin Ning |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biology Bioinformatics medicine.disease_cause Proteomics lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine proteomics bioinformatics biomarker inflammation microrna mitochondria mouse pathway analysis spinal cord injury stathmin Developmental Neuroscience medicine Amyotrophic lateral sclerosis Spinal cord injury Gene knockout lcsh:Neurology. Diseases of the nervous system Gene knockdown microRNA Cell redox homeostasis medicine.disease 030104 developmental biology Biomarker (medicine) Corrigendum 030217 neurology & neurosurgery Oxidative stress Research Article |
| Zdroj: | Neural Regeneration Research, Vol 15, Iss 6, Pp 1102-1110 (2020) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| Popis: | Our previous study found that microRNA-21a-5p (miR-21a-5p) knockdown could improve the recovery of motor function after spinal cord injury in a mouse model, but the precise molecular mechanism remains poorly understood. In this study, a modified Allen's weight drop was used to establish a mouse model of spinal cord injury. A proteomics approach was used to understand the role of differential protein expression with miR-21a-5p knockdown, using a mouse model of spinal cord injury without gene knockout as a negative control group. We found that after introducing miR-21a-5p knockdown, proteins that played an essential role in the regulation of inflammatory processes, cell protection against oxidative stress, cell redox homeostasis, and cell maintenance were upregulated compared with the negative control group. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis identified enriched pathways in both groups, such as the oxidative phosphorylation pathway, which is relevant to Parkinson's disease, Huntington's disease, Alzheimer's disease, and cardiac muscle contraction. We also found that miR-21a-5p could be a potential biomarker for amyotrophic lateral sclerosis, as miR-21a-5p becomes deregulated in this pathway. These results indicate successful detection of some important proteins that play potential roles in spinal cord injury. Elucidating the relationship between these proteins and the recovery of spinal cord injury will provide a reference for future research of spinal cord injury biomarkers. All experimental procedures and protocols were approved by the Experimental Animal Ethics Committee of Shandong University of China on March 5, 2014. |
| Databáze: | OpenAIRE |
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