Local Administration of Gold Nanoparticles Prevents Pivotal Pathological Changes in Murine Models of Atopic Asthma
| Autor: | Rafael Vital dos Santos, Suelen G. Trindade, Amanda C. Cotias, Cássio E. A. Santos, Vinicius F. Carvalho, Renato S.B. Cordeiro, Jandir M. Hickmann, Emiliano Barreto, Marco A. Martins, Patrícia M.R. e Silva, Cristiano Giacomelli, Vanessa Schimidt, Magda F. Serra, Camila Ribeiro Rodrigues Pão |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Chemokine medicine.medical_treatment Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Metal Nanoparticles Bioengineering Inflammation Injections Intralesional medicine.disease_cause Thiobarbituric Acid Reactive Substances Mice Fibrosis medicine Animals General Materials Science biology business.industry Pneumonia respiratory system medicine.disease Mucus Asthma respiratory tract diseases Ovalbumin Disease Models Animal Cytokine Neutrophil Infiltration Colloidal gold Immunology biology.protein Disease Progression Gold medicine.symptom business Reactive Oxygen Species Oxidative stress |
| Zdroj: | Journal of biomedical nanotechnology. 11(6) |
| ISSN: | 1550-7033 |
| Popis: | Although gold nanoparticles have been shown to exhibit a range of beneficial biological properties, including antiinflammatory and anti-oxidant effects, their putative impact on allergic asthma has not been addressed. In this study, we evaluated the potential of nasal-instilled gold nanoparticles to prevent allergen-induced asthma in distinct murine models of this disease. Swiss-Webster (outbred) and A/J (inbred) mice were sensitized with ovalbumin and then treated with intranasal injections of gold nanoparticles (6 and 60 μg/kg), 1 h before ovalbumin challenges. Lung function, leukocyte infiltration, mucus exacerbation, extracellular matrix deposition, cytokine generation and oxidative stress were evaluated 24 h after the last challenge. In both mice strains, gold nanoparticles clearly inhibited (70-100%) allergen-induced accumulation of inflammatory cells as well as the production of both pro-inflammatory cytokines and reactive oxygen species. In A/J mice, recognized as genetic asthma prone animals, instilled gold nanoparticles clearly prevented mucus production, peribronchiolar fibrosis and airway hyper-reactivity triggered by allergen provocation. In conclusion, these findings demonstrate that gold nanoparticles prevented pivotal features of asthma, including airway hyper-reactivity, inflammation and lung remodelling. Such protective effects are accounted for by reduction in lung tissue generation of pro-inflammatory cytokines and chemokines, in a mechanism probably related to down-regulation in the levels of oxidative stress. |
| Databáze: | OpenAIRE |
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