Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
| Autor: | Isabel García-Peláez, Felipe Cervantes-Sodi, Alejandra Contreras-Ramos, Emilio Rojas, Mahara Valverde, Luis A. Lara-Martínez, Eduardo Palacios González, Felipe Masso, Salomón Hernández-Gutiérrez |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
nanostructure
Biocompatibility Cell Biophysics Mice Nude Pharmaceutical Science Bioengineering Chick Embryo 02 engineering and technology scaffold 010402 general chemistry 01 natural sciences Biomaterials biocompatibility International Journal of Nanomedicine Toxicity Tests Drug Discovery medicine Animals Cytotoxicity Cells Cultured Original Research Nanotubes Carbon Cell growth Chemistry Cell Cycle Organic Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Cell cycle 021001 nanoscience & nanotechnology Rats 0104 chemical sciences Oxygen Transplantation Cell Transformation Neoplastic cell proliferation medicine.anatomical_structure Cancer cell Carcinogens 0210 nano-technology carcinogenic |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Luis A Lara-Martínez,1 Felipe Massó,2 Eduardo Palacios González,3 Isabel García-Peláez,4 Alejandra Contreras–Ramos,5 Mahara Valverde,6 Emilio Rojas,6 Felipe Cervantes-Sodi,7 Salomón Hernández-Gutiérrez1 1Department of Molecular Biology, School of Medicine, Universidad Panamericana, Mexico City, Mexico; 2Department of Physiology, National Institute of Cardiology Ignacio Chavez, Mexico City, Mexico; 3Department of Microscopy, Ultra High Resolution Electron Microscopy Laboratory, Instituto Mexicano del Petróleo, Mexico City, Mexico; 4Department of Embryology, Medicine Faculty, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico; 5Department of Developmental Biology Research and Experimental Teratogenicity, Children’s Hospital of Mexico, Federico Gomez, Mexico City, Mexico; 6Department of Genomic Medicine, Institute of Biomedical Research, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico; 7Department of Physics and Mathematics, Nanoscience and Nanotechnology Laboratory, Universidad Iberoamericana, Mexico City, Mexico Abstract: Carbon nanotubes (CNTs) have been a focus of attention due to their possible applications in medicine, by serving as scaffolds for cell growth and proliferation and improving mesenchymal cell transplantation and engraftment. The emphasis on the benefits of CNTs has been offset by the ample debate on the safety of nanotechnologies. In this study, we determine whether functionalized multiwalled CNTs (fMWCNTs) and functionalized oxygen-doped multiwalled CNTs (fCOxs) have toxic effects on rat mesenchymal stem cells (MSCs) in vitro by analyzing morphology and cell proliferation and, using in vivo models, whether they are able to transform MSCs in cancer cells or induce embryotoxicity. Our results demonstrate that there are statistically significant differences in cell proliferation and the cell cycle of MSCs in culture. We identified dramatic changes in cells that were treated with fMWCNTs. Our evaluation of the transformation to cancer cells and cytotoxicity process showed little effect. However, we found a severe embryotoxicity in chicken embryos that were treated with fMWCNTs, while fCOxs seem to exert cardioembryotoxicity and a discrete teratogenicity. Furthermore, it seems that the time of contact plays an important role during cell transformation and embryotoxicity. A single contact with fMWCNTs is not sufficient to transform cells in a short time; an exposure of fMWCNTs for 2weeks led to cell transformation risk and cardioembryotoxicity effects. Keywords: nanostructure, biocompatibility, scaffold, cell proliferation, cell cycle, carcinogenic |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|