Ultraviolet enhanced reactivation of a human virus: Effect of delayed infection
| Autor: | L E, Bockstahler, C D, Lytle, J E, Stafford, K F, Haynes |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Ultraviolet Rays
Health Toxicology and Mutagenesis Cell Viral Plaque Assay Biology Kidney medicine.disease_cause Virus Cell Line Herpes virus Monkey kidney Chlorocebus aethiops Genetics medicine Animals Humans Simplexvirus Irradiation Molecular Biology Dose-Response Relationship Radiation Virology Molecular biology medicine.anatomical_structure Herpes simplex virus Human Virus Ultraviolet |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 35:189-198 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(76)90184-6 |
| Popis: | The ability of UV-irradiated herpes simplex virus to form plaques was examined in monolayers of CV-1 monkey kidney cells preexposed to UV radiation at different intervals before virus assay. From analysis of UV reactivation (Weigle reactivation) curves it was found that as the interval between cell UV irradiation (0–20 J/m2) and initiation of the virus assay was increased over a period of five days, (1) the capacity of the cells to support unirradiated virus plaque formation, which was decreased immediately following UV exposure to the monolayers, increased and returned to approximately normal levels within five days, and (2) at five days an exponential increase was observed in the relative plaque formation of irradiated virus as a function of UV fluence to the monolayers. For high UV fluence (20 J/m2) to the cells, the relative plaque formation by the UV-irradiated virus at five days was about 10-fold higher than that obtained from assay on unirradiated cells. This enhancement in plaque formation is interpreted as a delayed expression of Weigle reactivation. The amount of enhancement resulting from this delayed reactivation was several fold greater than that produced by the Weigle reactivation which occurred when irradiated herpes virus was assayed immediately following cell irradiation. |
| Databáze: | OpenAIRE |
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