Enhanced histamine production through the induction of histidine decarboxylase expression by phorbol ester in Jurkat cells
| Autor: | Yusuke Nagashima, Akiyoshi Fukamizu, Jun-Dal Kim, Koichiro Kako |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight tandem mass spectrometry Histidine Decarboxylase Biochemistry Jurkat cells Jurkat Cells chemistry.chemical_compound Genetics Humans RNA Messenger Molecular Biology Chromatography High Pressure Liquid Histamine Production Articles histamine Histidine decarboxylase Molecular biology Up-Regulation Oncology chemistry ultra-high performance liquid chromatography Apoptosis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Tetradecanoylphorbol Acetate Quinolines Phorbol Molecular Medicine Histamine Intracellular phorbol 12-myristate 13-acetate |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2012.1049 |
| Popis: | Histamine (HA), a mediator of inflammation, type I allergic responses and neurotransmission, is synthesized from L-histidine, the reaction of which is catalyzed by histidine decarboxylase (HDC). HDC has been reported to be induced by various stimuli, not only in mast cells and basophils, but also in T lymphocytes and macrophages. Although its mRNA has been shown to be increased in Jurkat cells when treated with phorbol 12-myristate 13-acetate (TPA), little is known concerning the induced production of HA by HDC. The present study quantified the trace amounts of intracellular HA using ultra-high liquid chromatography in combination with the 6-aminoquinoline carbamate-derivatization technique. To test whether the cellular level of HA is elevated by the induction of HDC in Jurkat cells treated with TPA, the peak corresponding to authentic HA in the cell lysate was fractioned and its molecular weight determined by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. The results of this study show that the HA level is increased by the induction of HDC expression by TPA in Jurkat cells. Therefore, this method is useful in elucidating the physiological significance of HA production. |
| Databáze: | OpenAIRE |
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