High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment
| Autor: | Shengying Liang, Fangfang Cheng, Fen Yang, Juntang Lin, Yakun Ren, Lihong Guan, Yuliang Sun, Yanan He, Yanli Liu |
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| Rok vydání: | 2019 |
| Předmět: |
Lysis
QH301-705.5 Science Biology Endometrium General Biochemistry Genetics and Molecular Biology Andrology 03 medical and health sciences 0302 clinical medicine medicine Biology (General) 030304 developmental biology Differential centrifugation Adult stem cells 0303 health sciences Stem cell based therapy Transplantation Red blood cell High-yield medicine.anatomical_structure Menstrual blood-derived endometrial stem cells Subculture (biology) Stem cell General Agricultural and Biological Sciences Red blood cell lysis 030217 neurology & neurosurgery Adult stem cell Methods and Techniques |
| Zdroj: | Biology Open Biology Open, Vol 8, Iss 5 (2019) |
| ISSN: | 2046-6390 |
| Popis: | Recently, menstrual blood-derived endometrial stem cells (MenSCs) have become attractive for stem cell based therapy due to their abundance, easy and non-invasive extraction and isolation process, high proliferative capacity, and multi-lineage differentiation potential. MenSC-based therapies for various diseases are being extensively researched. However, the high death rate and poor engraftment in sites of damaged tissues reduce the therapeutic value of these stem cells for transplantation. In theory, periodic stem cell transplantation is an alternative strategy to overcome the challenge of the loss of beneficial stem cell-derived effects due to the rapid disappearance of the stem cells in vivo. However, periodic stem cell transplantation requires sufficient amounts of the desired stem cells with a low number of subculture passages. Our previous results have demonstrated that primary MenSCs mainly reside in the deciduous endometrium, and considerable amounts of deciduous endometrium intertwined with menstrual blood clots were discarded after conventional density gradient centrifugation (DGC). Therefore, the aim of this study was to determine whether primary MenSCs exist in the sedimentation of the deciduous endometrium after DGC and further to evaluate the isolation of MenSCs by direct red blood cell lysis treatment. As expected, our results confirmed that substantial amounts of primary MenSCs still remain in the sedimentation after DGC and indicated that MenSC isolation by directly lysing the red blood cells not only guaranteed substantial amounts of superior MenSCs with a low number of subculture passages, but also was time efficient and economical, providing a solid support for extensive clinical application. Summary: MenSC isolation by directly lysing the red blood cells not only guarantees substantial amounts of superior MenSCs with low passage number, but also is time efficient and economical. |
| Databáze: | OpenAIRE |
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