Immunolocalization of an Amino-Terminal Fragment of Apolipoprotein E in the Pick's Disease Brain
| Autor: | Raquel J. Brown, Ryan J. Day, Lindsey W. Catlin, Wayne W. Poon, Alexander J. Rajic, Troy T. Rohn |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Apolipoprotein E Pathology medicine.medical_specialty lcsh:Medicine Pathogenesis 03 medical and health sciences Apolipoproteins E 0302 clinical medicine Pick Disease of the Brain medicine Humans lcsh:Science CA1 Region Hippocampal 030304 developmental biology 0303 health sciences Multidisciplinary biology Dentate gyrus lcsh:R medicine.disease Molecular biology 3. Good health Dentate Gyrus biology.protein Immunohistochemistry lcsh:Q Pick's disease Antibody Alzheimer's disease 030217 neurology & neurosurgery Research Article Frontotemporal dementia |
| Zdroj: | PLoS ONE, Vol 8, Iss 12, p e80180 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0080180 |
| Popis: | Although the risk factor for apolipoprotein E (apoE) polymorphism in Alzheimer's disease (AD) has been well described, the role that apoE plays in other neurodegenerative diseases, including Pick's disease, is not well established. To examine a possible role of apoE in Pick's disease, an immunohistochemical analysis was performed utilizing a novel site-directed antibody that is specific for an amino-terminal fragment of apoE. Application of this antibody, termed the amino-terminal apoE cleavage fragment (nApoECF) antibody, consistently labeled Pick bodies within area CA1 of the hippocampus in 4 of the 5 cases examined. Co-localization of the nApoECF antibody with PHF-1, a general marker for Pick bodies, as well as with an antibody to caspase-cleaved tau (TauC3) was evident within the hippocampus. While staining of the nApoECF antibody was robust in area CA1, little co-localization with PHF-1 in Pick bodies within the dentate gyrus was observed. A quantitative analysis indicated that approximately 86% of the Pick bodies identified in area CA1 labeled with the nApoECF antibody. The presence of truncated apoE within Pick bodies suggests a broader role of apoE beyond AD and raises the question as to whether this protein contributes to pathogenesis associated with Pick's disease. |
| Databáze: | OpenAIRE |
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