Effect of extender composition and equilibration time on fertilization ability and enzymatic activity of rainbow trout cryopreserved spermatozoa
| Autor: | Stefan Dobosz, W. Demianowicz, H. Kuzminski, K. Goryczko, Igor Babiak, Jerzy Strzeżek, Jan Glogowski |
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| Rok vydání: | 2001 |
| Předmět: |
Male
endocrine system Time Factors food.ingredient Acid Phosphatase Semen Cryopreservation law.invention Andrology Cryoprotective Agents food Human fertilization Food Animals law Yolk Acetamides Animals Aspartate Aminotransferases Small Animals L-Lactate Dehydrogenase biology urogenital system Equine Extender biology.organism_classification Spermatozoa Sperm Trout Biochemistry Fertilization Oncorhynchus mykiss Sperm Motility Female Animal Science and Zoology Rainbow trout Semen Preservation |
| Zdroj: | Theriogenology. 56:177-192 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/s0093-691x(01)00553-2 |
| Popis: | The effects of extender composition and equilibration time on fertilizing ability of cryopreserved spermatozoa from rainbow trout, Oncorhynchus mykiss, were investigated. In addition, enzyme activity in supernatants from thawed sperm was assessed. The use of the two extenders: Erdahl & Graham's + 10% DMA (dimethyl acetamide) + 10% egg yolk and 0.3 M glucose + 10% DMA yielded the highest post-thaw fertilization rates. We observed interactions between extender constituents and the equilibration of diluted semen. This indicates a multifactorial effect of the extender constituents on spermatozoal resistance against injuries. The 10-min equilibration of spermatozoa in extender before freezing generally lowered the fertilization ability of spermatozoa, except for DMA-based extenders. The addition of egg yolk to the extender was generally beneficial, especially in DMA- and DMSO-based extenders. The use of low-density lipoprotein fraction showed no advantage to full-yolk or free-of-yolk extenders. Aspartate aminotransferase and lactate dehydrogenase leakage from damaged spermatozoa correlated negatively with the ability of cryopreserved spermatozoa to fertilize eggs. Each factor tested, when analyzed separately, did not give general information about its effect on the fertilization ability of cryopreserved sperm. The multifactorial analysis of the important factors in cryopreservation of trout spermatozoa showed their cumulative effect. This is the most likely reason for divergent information reported elsewhere on the effect of various factors in the cryopreservation of rainbow trout spermatozoa. |
| Databáze: | OpenAIRE |
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