Improved immunogenicity of a tuberculosis DNA vaccine encoding ESAT6 by DNA priming and protein boosting
| Autor: | Qing-min Wang, Jian-cheng Zhang, Ming Yin, Cun-jie Xiao, Zhen-lin Hu, Shuhan Sun |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_treatment
Immunization Secondary Enzyme-Linked Immunosorbent Assay Transfection Cell Line DNA vaccination Interferon-gamma Mice chemistry.chemical_compound Immune system Adjuvants Immunologic Bacterial Proteins Antigen Vaccines DNA medicine Animals Lymphocytes Tuberculosis Vaccines Antigens Bacterial Immunity Cellular Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health Th1 Cells Antibodies Bacterial Immunohistochemistry Virology Vaccination Infectious Diseases chemistry Immunoglobulin G Immunology biology.protein Molecular Medicine Female Interleukin-4 Antibody Adjuvant Cell Division Spleen DNA Plasmids |
| Zdroj: | Vaccine. 22:3622-3627 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2004.03.029 |
| Popis: | The study evaluated the immune response elicited by a DNA vaccine encoding ESAT6 protein of Mycobacterium tuberculosis by DNA prime-protein boost protocol. BALB/c mice were respectively vaccinated with plasmid DNA encoding ESAT6 protein, with ESAT6 protein in IFA adjuvant, or a combined DNA prime-protein boost regimen. While DNA immunization induced Th1-polarized immune response, protein–in-adjuvant vaccination elicited a Th2-dominant response. When animals were primed with DNA and boost with protein, both antibodies and Th-cell proliferative response were significantly enhanced. Moreover, production of Th1-type cytokine (IFN-γ) was increased significantly by DNA priming-protein boosting. This protocol also resulted in an increased relative ratio of IgG2a to IgG1 and the cytotoxicity of T cells. Thus, this study demonstrated that the formation of ESAT6 DNA prime-protein boost inoculation could improved antigen-specific cellular immune responses, which are important for protection against TB infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect