Immature T lymphocytes in human cord blood identified by monoclonal antibodies: a model for the study of the differentiation pathway of T cells in humans
| Autor: | M. L. Ferrando, Maria Teresa Fierro, M. C. Giubellino, Robert Foa, P. Lusso |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class T-Lymphocytes Immunology Antibodies Monoclonal Fluorescent Antibody Technique Cell Differentiation Biology Monoclonal antibody Fetal Blood Phenotype Andrology Antigen Cord blood Precursor cell Antigens Surface medicine Cytotoxic T cell Common Thymocyte Humans Clone (B-cell biology) |
| Zdroj: | Cellular immunology. 89(1) |
| ISSN: | 0008-8749 |
| Popis: | The reactivity of human cord blood lymphocytes was assessed against a panel of monoclonal antibodies (MoAb). The mean proportion of OKT3+ cells (pan-T) was significantly lower in cord blood (52 ± 13.8%; mean ± SD) compared with that of adult blood (75 ± 8.9%) and paralleled well with the E-rosette-forming capacity (50 ± 16.3%). Both the proportions of OKT4+ cells (helper/inducer phenotype) and of OKT8+ cells (suppressor/cytotoxic phenotype) were significantly reduced in cord blood (43 ± 11.8% vs 50.3 ± 7.4% and 20 ± 10.3% vs 25.6 ± 6.0%, respectively), while the overall OKT 4 OKT 8 ratio was increased compared with adult blood (2.87 ± 1.83 vs 2.04 ± 0.61). Unlike adult blood, in 30 of the 35 samples of cord blood an overlap was observed between the total proportion of OKT4+ and OKT8+ cells (65 ± 15.2%) and that of OKT3+ cells (52 ± 14.3%). Although small numbers of cells coexpressing both antigens were occasionally found, double-staining analysis showed that the overlap in cord blood was mostly due to an expanded proportion of OKT 3 ( Leu -4) − OKT 8 ( Leu -2) + cells. Relevant proportions of OKT6+ (common thymocyte antigen) and OKT10+ (thymocytes, activated T cells, precursor cells) cells were found in cord blood as opposed to adult blood (10.8 ± 8.6% vs 0.6 ± 0.6% and 67 ± 18.0% vs 8 ± 2.1%, respectively), while terminal deoxynucleotidyl transferase-positive cells were observed only in two samples of cord blood. A small proportion of T cells (E-rosette+) reacted with the MoAb OKIa1 (HLA-DR). Finally, the proportion of cord blood cells recognized by the MoAb Leu-7 (HNK-1 clone) was almost negligible compared with adult blood (2.8 ± 2.4% vs 15 ± 7.5%). These data confirm the immaturity and heterogeneity of cord blood lymphocytes and demonstrate the presence at birth of circulating lymphocytes which express a surface phenotype reminiscent of that found in the late stages of intrathymic differentiation and in some human T-cell leukemias. Human cord blood may thus represent a suitable model for the study of the differentiation pathway of normal and pathological T-cells in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|