Increased Osteoblast and Osteoclast Activity in Female Senescence-Accelerated, Osteoporotic SAMP6 Mice During Fracture Healing
| Autor: | Tim Pohlemann, Moritz Klein, Claudia Scheuer, Joerg H. Holstein, Swantje Kuntz, Tina Histing, Patric Garcia, Andrea Tami, Michael D. Menger, David Stenger |
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| Rok vydání: | 2012 |
| Předmět: |
Male
musculoskeletal diseases Aging medicine.medical_specialty Deoxypyridinoline Acid Phosphatase Osteocalcin Osteoclasts Bone healing Bone remodeling Mice chemistry.chemical_compound Osteoprotegerin Osteoclast Internal medicine medicine Animals Amino Acids Bony Callus Tartrate-resistant acid phosphatase Fracture Healing Sex Characteristics Osteoblasts biology Tartrate-Resistant Acid Phosphatase business.industry Osteoblast Mice Mutant Strains Biomechanical Phenomena Isoenzymes Disease Models Animal medicine.anatomical_structure Endocrinology chemistry biology.protein Osteoporosis Female Surgery Bone Remodeling business |
| Zdroj: | Journal of Surgical Research. 175:271-277 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2011.03.052 |
| Popis: | Background Previous studies have shown that fracture healing depends on gender and that in females, ovariectomy-induced osteoporosis impairs the healing process. There is no information, however, whether the alteration of fracture healing in osteoporosis also depends on gender. Materials and Methods Therefore, we herein studied fracture healing in female and male senescence-accelerated osteoporotic mice, strain P6 (SAMP6), including biomechanical, histomorphometric, and protein biochemical analysis. Results Bending stiffness was reduced in male and female SAMP6 mice compared with senescence-resistant strain 1 (SAMR1) controls. This was associated with elevated serum concentrations of tartrate-resistent acid phosphatase form 5b (TRAP) in both female and male SAMP6 mice. Callus size, however, was significantly larger in female SAMP6 mice compared with male SAMP6 mice and female SAMR1 controls. This indicates a delayed remodeling process in female SAMP6 mice. The delay of callus remodeling in female SAMP6 mice was associated with a significantly higher osteoprotegerin (OPG) callus tissue expression and increased serum concentrations of osteocalcin (OC) and deoxypyridinoline (DPD), indicating elevated osteoblast and osteoclast activities. Conclusion The present study shows that remodeling during fracture healing in female, but not in male, SAMP6 mice is delayed, most probably due to an increased osteoblast and osteoclast activity. |
| Databáze: | OpenAIRE |
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