Prevalance, Morphology, and Molecular Characterization of Sarcocystis heydorni Sarcocysts from Cattle (Bos Taurus) in China
| Autor: | S. Huang, G. W. Esch, Xin-Wen Chen, T. Wen, Jun-Jie Hu, T. T. Liu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetic Markers Veterinary medicine China Morphology (linguistics) Sarcocystosis Prevalence Cattle Diseases Biology DNA Mitochondrial DNA Ribosomal 18S ribosomal RNA Microbiology 03 medical and health sciences Microscopy Electron Transmission RNA Ribosomal 18S Animals Humans Ecology Evolution Behavior and Systematics Feces Phylogeny Sarcocystis 030108 mycology & parasitology Ribosomal RNA DNA Protozoan biology.organism_classification Sarcocystis heydorni Cyclooxygenase 1 Parasitology Cattle Sarcocystis hominis RNA Protozoan |
| Zdroj: | The Journal of parasitology. 102(5) |
| ISSN: | 1937-2345 |
| Popis: | Cattle are intermediate hosts for 2 zoonotic species of Sarcocystis, Sarcocystis hominis and Sarcocystis heydorni. Here we report S. heydorni from cattle for the first time in China. Sarcocysts of S. heydorni were found in muscle from 173 of 1,630 (10.6%) cattle in abattoirs (9.7% in skeletal muscles, 3.4% esophagus, 2.5% diaphragm, and 0.1% tongue; heart muscle was negative). By means of light microscopy, S. heydorni sarcocysts were thin-walled (1 μm). Using transmission electron microscopy, the sarcocyst wall had short (0.3-0.5 × 0.5-0.9 μm) stubby protrusions, the tips of which contained electron-dense, disk-shaped plaques, similar to the sarcocyst wall type 29b. In preliminary transmission attempts, a human volunteer did not excrete sporocysts in feces after ingesting 579 sarcocysts S. heydorni isolated from cattle. Phylogenetic analysis using the 2 molecular markers (18S rRNA gene and mitochondrial cox1 gene) indicated S. heydorni shared the closest affinity with species of Sarcocystis, which employ ruminants as intermediate hosts and canids as definitive hosts. |
| Databáze: | OpenAIRE |
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