Green Fluorescent Protein-Based Biosensor To Detect and Quantify Stress Responses Induced by DNA-Degrading Colicins

Autor:	Huub J. M. Brouwers, Ren Zhang, James Chin, Bernadette Turner, Toni A. Chapman, Sam Abraham
Rok vydání:	2011
Předmět:	Transcription Genetic DNA damage Green Fluorescent Proteins Colicins Biosensing Techniques macromolecular substances Biology Applied Microbiology and Biotechnology Green fluorescent protein chemistry.chemical_compound SOS Response (Genetics) Bacteriocin Methods Escherichia coli SOS response Promoter Regions Genetic SOS Response Genetics Ecology technology industry and agriculture Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition Molecular biology chemistry Colicin Biophysics bacteria Biosensor DNA DNA Damage Food Science Biotechnology
Zdroj:	Applied and Environmental Microbiology. 77:6691-6693
ISSN:	1098-5336 0099-2240
DOI:	10.1128/aem.00534-11
Popis:	Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcb15fa003008903e929b4ce58e42da9 https://doi.org/10.1128/aem.00534-11 Zobrazit plný text záznamu