Detection of sperm-reactive antibodies in wild sika deer and identification of the sperm antigens
| Autor: | Osamu Kawase, Mitsuru Jimbo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
sperm-reactive antibody
Male Sterility sika deer Wildlife Science Animals Wild Cross Reactions Autoantigens law.invention Microbiology Feces Antigen law sperm antigen Animals Humans Cervus nippon Intestinal Mucosa Autoantibodies Gastrointestinal tract Cervus Full Paper General Veterinary biology Molecular mass Deer biology.organism_classification Spermatozoa Sperm Fertility biology.protein Recombinant DNA Antibody |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.17-0660 |
| Popis: | Antisperm antibodies potentially inhibit sperm functions causing the sterility in humans and experimentally treated animals. However, there is no information about antisperm antibodies emerging spontaneously in wildlife. In this study, we searched for the sperm-reactive antibodies, spontaneously produced in wild sika deer (Cervus nippon), and identified the sperm antigens. We collected 529 fecal masses of sika deer in Japanese cities, from which we extracted the mucosal antibodies to test them for reactivities to deer sperm proteins by ELISA. Two of the extracts contained IgAs that were highly reactive to the sperm proteins. The molecular weights of the active IgAs, partially purified by DEAE-sephadex A-50, were estimated at more than 100 kDa, suggesting that the IgAs evaded drastic digestion in the gastrointestinal tract. Two-dimensional electrophoresis and immunoblotting detected three major antigens, and the following LC-MS/MS analysis identified them as alpha-enolase, phosphoglycerate kinase 2 and acrosin-binding protein. The antibodies were cross-reactive to a recombinant human acrosin-binding protein. To our knowledge, this is the first research to find that the sperm-reactive antibodies are produced spontaneously in wildlife and they recognize a common antigen found in humans. |
| Databáze: | OpenAIRE |
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