Barbed channels enhance unidirectional connectivity between neuronal networks cultured on multi electrode arrays
| Autor: | Wim Rutten, Joost le Feber, Eddy L de Weerd, Wybren Postma, Marcel H.H. Weusthof |
|---|---|
| Přispěvatelé: | Faculty of Science and Technology, Clinical Neurophysiology |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Cellular level lcsh:RC321-571 IR-98611 electrical stimulation lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry spontaneous activity stimulus response Original Research business.industry cortical neurons General Neuroscience METIS-315074 Cortical neurons electrophysiology multi electrode array Axon growth Stimulus response EWI-26529 Electrophysiology stimulus response compatibility Electrode Optoelectronics BSS-Neurotechnology and cellular engineering business Neuroscience |
| Zdroj: | Frontiers in Neuroscience, 9(412), 00412-00412. Frontiers Research Foundation Frontiers in Neuroscience, Vol 9 (2015) Frontiers in Neuroscience |
| ISSN: | 1662-453X 1662-4548 |
| DOI: | 10.3389/fnins.2015.00412 |
| Popis: | Cultured neurons on multi electrode arrays (MEAs) have been widely used to study various as-pects of neuronal (network) functioning. A possible drawback of this approach is the lack of struc-ture in these networks. At the single cell level, several solutions have been proposed to enable di-rected connectivity, and promising results were obtained. At the level of connected sub-populations, a few attempts have been made with promising results. First assessment of the de-signs’ functionality, however, suggested room for further improvement. We designed a two chamber MEA aiming to create a unidirectional connection between the net-works in both chambers (‘emitting’ and ‘receiving’). To achieve this unidirectionality, all intercon-necting channels contained barbs that hindered axon growth in the opposite direction (from receiv-ing to emitting chamber). Visual inspection showed that axons predominantly grew through the channels in the promoted direction. This observation was confirmed by spontaneous activity re-cordings. Cross-correlation between the signals from two electrodes inside the channels suggested signal propagation at ≈2 m/s from emitting to receiving chamber. Cross-correlation between the fir-ing patterns in both chambers indicated that most correlated activity was initiated in the emitting chamber, which was also reflected by a significantly lower fraction of partial bursts (e. a one-chamber-only burst) in the emitting chamber. Finally, electrical stimulation in the emitting chamber induced a fast response in that chamber, and a slower response in the receiving chamber. Stimula-tion in the receiving chamber evoked a fast response in that chamber, but no response in the emit-ting chamber. These results confirm the predominantly unidirectional nature of the connecting channels from emitting to receiving chamber. |
| Databáze: | OpenAIRE |
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