Effect of pH, carbamylation and other hemoglobins on deoxyhemoglobin S aggregation inside intact erythrocytes as detected by proton relaxation rate measurements
| Autor: | G. Larry Cottam, Kotaro Yamaoka, A. Henry Chuang, Michael R. Waterman |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Heterozygote
Erythrocytes Magnetic Resonance Spectroscopy Proton Macromolecular Substances Protein Conformation Hemoglobins Abnormal Hemoglobin Sickle Biophysics Biochemistry Hemoglobins Nuclear magnetic resonance Humans Molecular Biology Deoxygenation Sickled erythrocytes Cyanates Deoxyhemoglobin S Binding Sites Chemistry Intermolecular force Hydrogen-Ion Concentration Oxygen Relaxation rate Oxyhemoglobins Relaxation (physics) Carbamates Maximum rate Protein Binding |
| Zdroj: | Archives of biochemistry and biophysics. 167(1) |
| ISSN: | 0003-9861 |
| Popis: | Measurement of the transverse water proton relaxation rate has been used to study the effect of pH, carbamylation, and other hemoglobins on the aggregation of deoxyhemoglobin S inside intact erythrocytes. Upon complete deoxygenation, cyanate-treated ( S S ) erythrocytes and erythrocytes heterozygous with respect to hemoglobin S ( A S , C S , and S D ) have high transverse water proton relaxation rates very similar to the values obtained with homozygous ( S S ) erythrocytes. These results suggest extensive intermolecular interactions between deoxyhemoglobin S molecules and a resultant increase in the correlation time for the small fraction of “irrotationally bound” water. When the transverse relaxation rate in deoxygenated ( S S ) erythrocytes was measured as a function of pH, the maximum rate was observed between pH 7.0 and 7.5. Upon increasing the pH beyond this range the observed relaxation rate decreases as does the number of sickled cells. Upon decreasing the pH, the observed transverse relaxation rate also decreases but the ratio of values from deoxy oxy ( S S ) erythrocytes remains in the normal range of 4–6 and the number of sickled cells does not change. Therefore, the deoxyhemoglobin S aggregate inside sickled erythrocytes, as observed by water proton relaxation rates, is not altered by carbamylation or by the presence of nongelling hemoglobins. In addition, the enhancement of the relaxation rates as a function of pH is consistent with the number of sickled forms observed. |
| Databáze: | OpenAIRE |
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