Adhesion-free cell migration by topography-based force transduction
| Autor: | I. d. de Vries, Jack Merrin, Robert Hauschild, Anne Reversat, Michael Sixt, Raphaël Voituriez, Andrew Callan-Jones, Matthieu Piel |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
biology Chemistry Shear force Integrin fungi Cell migration Adhesion Actin cytoskeleton Transmembrane protein Coupling (electronics) 03 medical and health sciences 0302 clinical medicine Biophysics biology.protein 030217 neurology & neurosurgery Actin 030304 developmental biology |
| DOI: | 10.1101/793919 |
| Popis: | Eukaryotic cells migrate by coupling the intracellular force of the actin cytoskeleton to the environment. While force-coupling is usually mediated by transmembrane adhesion receptors, especially these of the integrin family, amoeboid cells like leukocytes can migrate extremely fast despite very low adhesive forces1. We show that leukocytes cannot only migrate under low adhesion but indeed can transduce forces in the complete absence of transmembrane force coupling. When confined within three-dimensional environments, they use the topographic features of the substrate to propel themselves. Here, the retrograde flow of the actin cytoskeleton follows the texture of the substrate, creating shear forces sufficient to drive deformations towards the back of the cell. Notably, adhesion dependent and adhesion independent migration are not exclusive but rather variants of the same principle of coupling retrograde actin flow to the environment and thus can potentially operate simultaneously. As adhesion free migration is independent of the chemical composition of the environment it renders cells completely autonomous in their locomotive behavior. |
| Databáze: | OpenAIRE |
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