Effects of medium and substratum conditions on the rates of DNA synthesis in primary cultures of bile ductular epithelial cells
| Autor: | Alphonse E. Sirica, Georg A. Mathis |
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| Rok vydání: | 1990 |
| Předmět: |
Male
Clinical Biochemistry Plant Science Biology digestive system Epithelium Tissue culture Epidermal growth factor medicine Animals Hydroxyurea Cells Cultured Matrigel Epidermal Growth Factor DNA synthesis Cell Differentiation Epithelial Cells Serum Albumin Bovine DNA Cell Biology Molecular biology Rats Inbred F344 Culture Media Extracellular Matrix Fibronectins Rats Bile Ducts Intrahepatic medicine.anatomical_structure Biochemistry Cell culture Biliary tract Collagen Fetal bovine serum Biotechnology Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology. 26:113-118 |
| ISSN: | 1475-2689 0883-8364 |
| DOI: | 10.1007/bf02624101 |
| Popis: | Select medium and substratum conditions were investigated for their effects on semiconservative DNA synthesis in essentially pure primary cultures of bile ductular epithelial cells that were initially isolated from cholestatic rat livers at 6 to 10 wk after bile duct ligation. DNA synthesis in these cultured cells was serum-dependent, being at its highest level when the concentration of fetal bovine serum present in the medium was maintained at 10%. This serum-dependent DNA synthesis was completely inhibited when 10 mM hydroxyurea was also included in the medium, and bile ductular cells cultured in the continued presence of 1.0% fetal bovine serum showed only marginal DNA synthesis during 8 to 10 d of primary culture when compared with no-serum controls. Maximum rates of serum-dependent DNA synthesis were obtained when the bile ductular cells were cultured for 7 to 14 d on tissue culture plastic coated with obtained when the bile ductular cells were cultured for 7 to 14 d on tissue culture plastic coated with either fibronectin from bovine plasma or type I rat-tail collagen. Cells cultured on plastic coated with basement membrane Matrigel exhibited the lowest levels of DNA synthesis, whereas those on plastic alone had intermediate amounts. Furthermore, the addition of epidermal growth factor (50 ng·ml−1·d−1) to medium supplemented with 1.0% fetal bovine serum greatly enhanced the rate of DNA synthesis in bile ductular cells after 6 d in primary culture on type I collagen-coated plastic over that measured in solvent control cultures. These findings indicate that our bile ductular epithelial cell culture model is potentially useful in the study of biliary cell growth regulation and carcinogenesis. |
| Databáze: | OpenAIRE |
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