Insulin and Metformin Regulate Circulating and Adipose Tissue Chemerin
| Autor: | Jing Chen, Krzysztof C. Lewandowski, Bee K. Tan, Hendrik Lehnert, S Farhatullah, Jaspreet Kaur, Raghu Adya, D Heutling, Harpal S. Randeva, J. Paul O'Hare |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Adipose tissue CMKLR1 Young Adult Organ Culture Techniques Insulin resistance Internal medicine Internal Medicine medicine Humans Hypoglycemic Agents Insulin Chemerin Testosterone RNA Messenger Dose-Response Relationship Drug Estradiol biology Dehydroepiandrosterone Sulfate Reverse Transcriptase Polymerase Chain Reaction Androstenedione medicine.disease Polycystic ovary Metformin Up-Regulation Glucose Metabolism Endocrinology Adipose Tissue biology.protein Female Receptors Chemokine Original Article Insulin Resistance Omentum Polycystic Ovary Syndrome medicine.drug Hormone |
| Zdroj: | Diabetes |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | OBJECTIVE To assess chemerin levels and regulation in sera and adipose tissue from women with polycystic ovary syndrome (PCOS) and matched control subjects. RESEARCH DESIGN AND METHODS Real-time RT-PCR and Western blotting were used to assess mRNA and protein expression of chemerin. Serum chemerin was measured by enzyme-linked immunosorbent assay. We investigated the in vivo effects of insulin on serum chemerin levels via a prolonged insulin-glucose infusion. Ex vivo effects of insulin, metformin, and steroid hormones on adipose tissue chemerin protein production and secretion into conditioned media were assessed by Western blotting and enzyme-linked immunosorbent assay, respectively. RESULTS Serum chemerin, subcutaneous, and omental adipose tissue chemerin were significantly higher in women with PCOS (n = 14; P < 0.05, P < 0.01). Hyperinsulinemic induction in human subjects significantly increased serum chemerin levels (n = 6; P < 0.05, P < 0.01). In adipose tissue explants, insulin significantly increased (n = 6; P < 0.05, P < 0.01) whereas metformin significantly decreased (n = 6; P < 0.05, P < 0.01) chemerin protein production and secretion into conditioned media, respectively. After 6 months of metformin treatment, there was a significant decrease in serum chemerin (n = 21; P < 0.01). Importantly, changes in homeostasis model assessment–insulin resistance were predictive of changes in serum chemerin (P = 0.046). CONCLUSIONS Serum and adipose tissue chemerin levels are increased in women with PCOS and are upregulated by insulin. Metformin treatment decreases serum chemerin in these women. |
| Databáze: | OpenAIRE |
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