Genome-wide transcriptome profiling provides overwintering mechanism of Agropyron mongolicum
| Autor: | Li Xiaoquan, Dongyang Yu, Li Tingting, Jiancai Du, Bing Han |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine China Genome-wide transcriptome Overwintering mechanisms Genomics Plant Science Biology 01 natural sciences Genome Transcriptome 03 medical and health sciences Gene Expression Regulation Plant lcsh:Botany Gene expression Botany KEGG Agropyron Gene Phylogeny Overwintering Plant Proteins Genetics Regulation of gene expression Gene Expression Profiling Agropyron mongolicum lcsh:QK1-989 030104 developmental biology Perennial gramineous plants Metabolic Networks and Pathways Research Article 010606 plant biology & botany |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 17, Iss 1, Pp 1-13 (2017) |
| ISSN: | 1471-2229 |
| DOI: | 10.1186/s12870-017-1086-3 |
| Popis: | Background The mechanism of winter survival for perennials involves multiple levels of gene regulation, especially cold resistance. Agropyron mongolicum is one important perennial grass species, but there is little information regarding its overwintering mechanism. We performed a comprehensive transcriptomics study to evaluate global gene expression profiles regarding the winter survival of Agropyron mongolicum. A genome-wide gene expression analysis involving four different periods was identified. Twenty-eight coexpression modules with distinct patterns were performed for transcriptome profiling. Furthermore, differentially expressed genes (DEGs) and their functional characterization were defined using a genome database such as NT, NR, COG, and KEGG. Result A total of 79.6% of the unigenes were characterized to be involved in 136 metabolic pathways. In addition, the expression level of ABA receptor genes, regulation of transcription factors, and a coexpression network analysis were conducted using transcriptome data. We found that ABA receptors regulated downstream gene expression by activating bZIP and NAC transcription factors to improve cold resistance and winter survival. Conclusion This study provides comprehensive transcriptome data for the characterization of overwintering-related gene expression profiles in A. mongolicum. Genomics resources can help provide a better understanding of the overwintering mechanism for perennial gramineae species. By analyzing genome-wide expression patterns for the four key stages of tiller bud development, the functional characteristics of the DEGs were identified that participated in various metabolic pathways and have been shown to be strongly associated with cold tolerance. These results can be further exploited to determine the mechanism of overwintering in perennial gramineae species. Electronic supplementary material The online version of this article (doi:10.1186/s12870-017-1086-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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